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Read a Codon Wheel
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Genetic Coding: codon charts
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Chapter 16- How Genes Work
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BIOL 1140 Chapters 15-16 Review Worksheet KEY Define transcription Transcription is the process of creating RNA from DNA. Describe how only one strand of DNA is used as the template in transcription. Only one of the strands (3’-5’ strand) serves as a template; the other strand is unused. Identify the three steps of transcription and briefly what is happening in each step. Initiation - in this step, the enzyme *RNA polymerase attaches to a region of the gene called a promoter, and transcription starts Elongation – In elongation, the enzyme RNA polymerase zips along the DNA strand adding complementary nucleotides to the DN template. The newly made RNA is fed out the back of the enzyme, and the two DNA strands re-anneal (re-zip). Remember that adenine, guanine, and cytosine are used in making RNA, but uracil is used in place of thymine. Termination - in this step, RNA polymerase reaches a sequence of nucleotides on the DNA template called a terminator. RNA polymerase then detaches from the newly synthesized RNA and the DNA. *before RNA polymerase binds, a series of proteins called transcription factors first bind to the promoter. Once they bind, they “recruit” RNA polymerase to the promoter. The binding of RNA polymerase starts the transcription process Which of the following statements regarding transcription is not true? A. The three stages of transcription are initiation, elongation and termination B. The key enzyme responsible for transcription is RNA polymerase C. Transcription is the conversion of information from DNA nucleotides into RNA nucleotides D. Transcription in eukaryotes is regulated (in part) by the binding of transcription factors to the promoter E. All are true statements What is the name of the enzyme used in transcription? Where does this enzyme bind? RNA polymerase…it binds to the gene’s promoter (though it doesn’t bind directly to the DNA strand) Indicate if the following statements about codons, amino acids and the genetic code are true or false: ____True______ A codon is a three nucleotide sequence that codes for a specific amino acid ____ True ______ In the genetic code, a codon will code for only one amino acid; that is there is specificity on the genetic code. ____False______ Some codons consist of only two nucleotides. 🡪 all are three nucleotides _____False_____ Some amino acids are not specified by any codons. 🡪 all amino acids have their own set of codons ____ True ______ Some codons (“stop codons”) do not code for any amino acid. ____ True ______ More than one codon can code for the same amino acid; that is, there is redundancy in the genetic code. Define translation. Translation is the process of creating proteins from RNA. Match the key players in translation with their function Answers B_____ tRNA A) The kind of RNA that makes up a ribosome. D______ mRNA B) Acts as the ‘interpreter’ in translation, by bringing in amino acids. C_______ DNA C) The genetic information in a cell. A_______ rRNA D) The kind of RNA that codes for amino acids. E_______ Ribosome E) Where translation takes place. Identify the three steps of translation and briefly describe what is happening in each step. Initiation – First an mRNA molecule binds to the small ribosomal subunit. A special initiator tRNA binds to a specific codon called the start codon (AUG = methionine). The initiator tRNA which carries the amino acid methionine, binds its anticodon (UAC) to the start codon . Second, a large ribosomal subunit binds to the small one, creating a function ribosome. The initiator tRNA binds to one of two tRNA binding sites on the ribosome. Elongation - In elongation amino acids are added one-by-one to the first amino acid. Each addition occurs in a three-step process: 1st: the anticodon of an incoming tRNA molecule, carrying its amino acid, pairs with the mRNA codon. 2nd: the incoming amino acid attaches by peptide bond to the amino acid already present – the formation of the bond is catalyzed by the ribosome. 3rd : The tRNA already present moves over to the next site (the codon and anticodon remain hydrogen bonded and the mRNA and tRNA move over as a unit), allowing another tRNA to move in. The second amino acid is then added to the growing polypeptide chain. The process is repeated - the first tRNA leaves the ribosome, the second tRNA moves over, allowing room for the next to move in…the process is repeated over and over again Termination - Elongation continues until a stop codon reaches the ribosome (recall that a stop codon does not code for any amino acid). The completed polypeptide is freed from the tRNA and the ribosome splits back into two separate subunits. Which of the following statements regarding translation is not true? A. Translation is the conversion of information from nucleic acids to proteins B. Translation takes place in the nucleus C. During translation, amino acids are linked to one another by peptide bonds D. Polypeptides made during translation must still be modified to become fully-functioning mature proteins E. All are true statements
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Colonic Cancer Notes
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Molecular Genetics Study Guide What is the Central Dogma of Molecular Genetics? The central dogma explains the flow of genetic information: DNA → RNA → Protein. What is transcription? The process of making an RNA copy (mRNA) from a DNA template. What is RNA polymerase? An enzyme that synthesizes RNA using a DNA strand as a template. What are promoter and terminator sequences? Promoter: DNA sequence where RNA polymerase binds to begin transcription. Terminator: DNA sequence signaling the end of transcription. What is messenger RNA (mRNA)? Carries genetic information from DNA to ribosomes for protein synthesis. How do DNA and RNA differ? DNA: Double-stranded, contains deoxyribose, bases A-T-C-G. RNA: Single-stranded, contains ribose, bases A-U-C-G (Uracil replaces Thymine). What is translation? Process of converting mRNA into a protein at the ribosome. What are codons? How many codons are there? Three-nucleotide sequences on mRNA that specify amino acids. There are 64 codons total. What are start and stop codons? Why necessary? Start: AUG (codes for Methionine) – signals where translation begins. Stop: UAA, UAG, UGA – signals end of translation. What is transferRNA (tRNA)? Brings specific amino acids to the ribosome according to the codons in mRNA. What are the roles of mRNA, ribosomes, and tRNA during translation? - mRNA: provides the template. - Ribosomes: site of protein synthesis. - tRNA: brings amino acids and matches them to the codon. What is an operon? Who discovered operons? A group of genes under the control of one promoter. Discovered by François Jacob and Jacques Monod. How do inducible and repressible operons differ? - Inducible: usually off; turned on by a molecule (e.g., Lac operon). - Repressible: usually on; turned off by a molecule (e.g., Trp operon). How does the Lac operon work? An inducible operon turned on when lactose is present. Lactose binds the repressor, freeing the operator for transcription. What are repressor proteins? What are operator sequences? Repressors: proteins that bind to the operator to block transcription. Operator: DNA region where repressors bind. What is a mutation? Examples of mutagens? A change in DNA sequence. Mutagens: UV light, chemicals, radiation. What are point mutations? Single base changes (e.g., substitution of one nucleotide). What are frame-shift errors? Insertions or deletions that shift the reading frame, affecting all downstream codons. How does UV radiation mutate DNA Causes thymine dimers (T-T bonds), distorting DNA and interfering with replication
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colon cancer
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APUSH Unit 1
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Chapter 19 Evaluation – Apply to all chapters – Once you implement a intervention -you need to assess whether it worked or not---- equals = EVALUATE whether it worked, --- Do I need to change the intervention, do I need to modify the intervention or do I say it worked = met the evaluation -- I achieved the outcome I was looking for. Chapter 20- Read over the terminology at the beginning. Very important terms. What does it mean to document and why is it so important? What does the patient record contain? What are the characteristics of effective documentation? What is the purpose of privacy and confidentiality? What happens if you do not follow those rules? READ over Box-20-1, what does HIPAA stand for? Glance at the abbreviations but we will go over !! Delegation is important when it comes to documentation - working with Nursing assistants - must follow all policies of what NA can chart/ LVN's and what the RN will chart . Need to know job description and duties!! For quality performance improvement how important is documentation? Types /Methods of documentation - Electronic EHR records, source oriented records, problem- oriented medical records = SOAP charting , PIE, focus charting, charting by exception, . Models of charting = CASE Management, collaborative, unusually occurrence, … Nursing documentation - Assessments, care plan, patient care summary - area charting- critical care, progress notes, flow sheet=graphic record. MAR, acquity, discharge/transfer, Home health documentation AMA, Nurses reporting to each other at the change of shift = change of shift report=Handoff reports , calling doctors, transferring patients < Incident reports. Chapter 30 -Medication Chapter: Administering medications is very important- understanding terminology is first- what do all the terminology mean and why? Pharmacology, pharmokinetics, pharmacist( knowing their responsibility - Prinicipals of pharmacology what/why do we need to understand nomenclature of drugs? Generic vs. trade, What are the different types of drugs ? why is that important? What is the importance of drug classification? Again what is Drug indications mean? Versus action of drug? How do the mechanics of drugs interfer with drug action? Pharmokinetics! What does the word Bioavailability mean? What are the different types of Drug reactions and how do they differ?( adverse, allergic, toxic, idiosyncratic). What type of factors can affect Drug action: ( developmental, weight, cultural/genetic, psychological, environmental, timing)- More terminology – half life, why do we need to know that? Can you give an example of that? Peak and trough -again what is the importance of collecting a peak and trough and demonstrate or explain how to do both? Principles of medication- medications prescriptions and orders. You need to know what an order is , what is included on an order what are the details of importance about the order ( what are the components on the order). What are different types of orders and can you describe the difference of how they work? Who can write orders? Are there rules to for checking orders and questioning orders? Know your math for medication . what are the rights to medication( there are a lot but ) which are the most important up front. Now, apply the Nursing Process to this chapter- what type of problems can the patient demonstrate while starting med, taking meds or . Here is a question-"Pharmacokinetics question type" – medication administered that are renal toxic should have what frequent assessment of which blood values: WBC, Ptt, BUN , creatinine, glucose. chapter 33 Skin/wounds- What is the function of the skin, layers of the skin- after that understand why I am asking you to know those functions and sites. Because you need to think how deep and where the wound is and what it looks like. Assess the wound : type clean/dirty, acute/chronic, intentional/unintentiona. How does a wound heal: phases of healing,- look at each phase- and recognize a specific details to know about that phase. What are complications of wounds? What do they mean? And if they happen what will the nurse do to assist the patient to over come their complication. What are factors to develop wounds? What different factors that can actually create or assist in the development of a wound? What do you look for when you are ASSESSING-(patient responses)- color, size, smell, location, drainage =color-what are colors and names of drainage? , amount, smell, - terminology-maceration, evisceration, fistula, tunneling, - . Pressure wounds- what are they? how do they develop? How do you know it is a pressure wound? Are there stages of a pressure wound? How do you tell the difference- again, look at specific detail!! ( earlier I asked you about the layers of the skin)!!!! How do we take care to of pressure ulces (wounds)???? What are some Nursing Interventions that can be done by nurse to prevent pressure wounds and how do care for them once they have developed and how do we slow them down to not progress to the next stage.?? Critical think -- what is important to take care of a wound. How do help people heal a wound. Can you create a pressure wound in 1-day??What are some colors of a pressure wound? What do the colors mean? If we need to debride a wound-- what does debridement mean? What types of debridement are there? ( autolytic, mechanical, enzymatic, sharp) --How do you know a wound is infected? What does a nurse need to do to check for an infection? How do they collect a specimen. lastly , Utilizing the Nursing process in this chapter-- and referring to Chapter 15 also -- when you have skin broken--- what type of Nursing diagnosis would you use to label the patients wound response???? Look over the different type of Nursing diagnosis. How does Heat and Cold help a patient and what problems could develop from either heat or cold. Chapter-text-37 Nutrition Chapter- what is the purpose of reading the labels on store items, what is the GI tract function? What is the function of the esophagus , stomach, small intestine, large colon, rectum and anus? What does a nutrient mean? How much of fat, Carbs , protein, ? Food label look it over. Look over the PLATE Food!! Why is nutrition so important? How does it help the patient? Why is this an important intervention to assess. How do you assess a patients nutritional status? What does Positive or Negative Nitrogen Balance mean? Anorexia Nervosus means what ? Anorexia means what ? Bulemia? What is malnutrition? How much weight can you lose that is steady and good to lose? Look at some labs: Albumin, Prealbumin, Cholesterol, triglycerides, CBC, SMA7=Na+, K+, cl, co2, gluc BUN, CREAT, Mg, CA. : Types of Diet- NPO, Ice chips, clear liquid, full liquid, puree diet, mechanical diet, - renal diet, liver diet, cardiac diet- what is included in the diet ( each of these diets) ie- what can you eat to get your potassium? To give you eyes nutrition? Pharmacology Book: (enteral/Parenteral chapter)-first what is the difference between enteral and parenteral nutrition? Under Enteral Nutrition- what is a the purpose of a salem sump tube versus a soft silastic tube with a wire in it? What is the primary purpose for each tube. What is the direction of how to place an nasogastric tube in position? How do we verifiy it is in the correct position. What are some management of care towards the nasogastric tube. What are some problems/complications having a nasogastic tube. Ok , we started tube feeding, what are some complications with starting tube feeding to someone who hasn’t had any food in awhile . If there is any complications from tube feeding how do you handle them? What is Parenteral Nutrition ? What is PPN vs. TPN. What is the primary ingredient in the PPN or the TPN? What are complications with TPN nutrition? How does Enteral Nutrition compare to Parenteral (TPN) Nutrition? What are some complications from TPN nutrition? Pharmacology Book : Vitamins/minerals vitamins and minerals - fat soluble and water soluble how do they differ? What is important about each vitamin? What should you teach your patient about these meds. Which meds can be toxic? What is important about IRON, what is important about Ferrous . How did the two differ? ( read very carefully for the difference)
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coronal polishing
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Disease of the Colon/Rectum
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Colon and Mesenteries
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BIOL 102 Unit 3 Codon Vocab
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Discovery/Colonization Vocab
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Reading a Codon Chart
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