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Fall 23 Final Exam Study Guide: Microbiology for Health Professionals • Specimen Collection o When is the best time to collect a specimen for culture? o Sterile containers vs nonsterile containers o What information is needed when labeling a specimen? o Most common source of contamination and how contamination usually come about in these samples in a hospital setting. • Media and Biochemical Tests: BAP, MAC, indole, TSI, coagulase o Know the difference between nonselective, selective, and differential media. o Explain how the appropriate media is chosen when plating specimens. o Whether plates are nonselective, selective, and differential as well as what they are selective/differential for o Know how to determine between a positive and negative result when preforming a biochemical test. o How each bacterium we used in class responds/looks on different plates and biochemical tests • Streaking for isolation o Explain the purpose of streaking for isolation. • Antibiotic Susceptibility Testing o What the Kirby Bauer Test is and how it demonstrated antibiotic Susceptibility, o How you would choose an antibiotic to treat an infection when considering the Kirby Bauer test results o Ways to prevent antibiotics resistance: finishing the entire prescription of antibiotics, not sharing antibiotics with others, only prescribing during an infection (seek professionals/don’t self-medicate and share), proper food prep, learning about antibiotic resistance and advocacy/teaching others • Case Study o Be able to identify the following organism when given the growth on media and biochemical test results: ▪ E. coli ▪ P. vulgaris ▪ K. pneumoniae ▪ Shigella sp

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