Molecular Genetics Chapter 11 - DNA Replication

The complementarity of DNA strands is based on

A. the chemical properties of a phosphodiester linkage

B. the binding of proteins to the DNA

C. the AT/GC rule

D. none of the above

C. the AT/GC rule

To make a new DNA strand, which of the following is/are necessary?

A. a template strand

B. nucleotides

C. heavy nitrogen

D. both A and B

D. a template strand and nucleotides

The model that correctly describes the process of DNA replication is

A. the conservative model

B. the semiconservative model

C. the dispersive model

D. all of the above models describe the process correctly

B. the semiconservative model

A site in a chromosome where DNA replication begins is

A. a promoter

B. an origin of replication

C. an operator

D. a replication fork

B. an origin of replication

The origin of replication in E. coli contains

A. an AT-rich region

B. DnaA boxes

C. GATC methylation sites

D. all of the above

D. an AT-rich region, DnaA boxes, and GATC methylation sites

Is DNA strand breakage necessary for catenane separation?

Yes because they can move relative to each other and the catenanes can separate from each other

The enzyme known as_______ uses _______ and separates the DNA strands at the replication fork

A. DNA helicase, ATP

B. DNA helicase, GTP

C. DNA gyrase, ATP

D. DNA gyrase, GTP

A. DNA helicase, ATP

In the lagging strand, DNA is made in the direction _______ the replication fork and is made as __________.

A. toward, one continuous strand

B. away from, one continuous strand

C. toward, Okazaki fragments

D. away from, Okazaki fragments

D. away from, Okazaki fragments

DNA polymerase III is a processive enzyme, which means that

A. it does not dissociate from the template strand after it has attached a nucleotide to the 3’ end of the daughter strand

B. it makes. anew strand very quickly

C. it proceeds toward the opening of the replication fork

D. it copies DNA with relatively few errors

A. it does not dissociate from the template strand after it has attached a nucleotide to the 3’ end of the daughter strand

The proofreading function of DNA polymerase involves the recognition of a _____ and the removal of a short segment of DNA in the _____ direction.

A. missing base, 5’ to 3’

B. base pair mismatch, 5’ to 3’

C. missing base, 3’ to 5’

D. base pair mismatch, 3’ to 5’

D. base pair mismatch, 3’ to 5’

In eukaryotes, DNA replication is initiated at an origin of replication by

A. DnaA proteins

B. the origin recognition complex

C. DNA polymerase

D. MCM helicase

B. the origin replication complex

In eukaryotes, RNA primers are primarily removed by

A. DNA polymerase I

B. DNA polymerase a

C. flap endonuclease

D. helicase

C. flap endonuclease

To synthesize DNA, what does telomerase use as a template?

A. it uses the DNA in the 3’ overhand region

B. it uses RNA that is a component of telomerase

C. no template is used

D. both A and B are correct

B. it uses RNA that is the component of telomerase