anatomy lab : microscopes

rules for proper microscope use

-carrying with two hands (one on arm & one on base)

-keep microscope upright

-keep lens clean

-use only the fine-focus knob when using high-power objective lens

higher magnification

results in smaller FOV

⭐️less area, more detail⭐️

lower magnification

results in wider FOV

⭐️more area, less detail⭐️

microscope diagram

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calculation total magnification

eyepiece (ocular) magnification X low-power objective magnification

ex → 10X x 4X + 40X

what happens to the depth of field with increasing magnification?

field of view decreases

iris diaphragm function

-controls the amount of light reaching the specimen

coverslip

thin glass plate placed on top of specimen

microscope we use in lab

compound light microscope

name of circle you see when you look through the ocular lens of the microscope

field of view (FOV)

when should you use the scanning lens on a microscope?

to get a broad overview of the slide & locate your specimen

how should you clean the lens of a microscope?

70% alcohol or distilled wate

FOV to determine size of object under microscope

Object Size = Field of View Diameter

÷divided by ÷ Number of Objects (Fit Number).