genetics - exam #3

what is the triplet code of mRNA and complementary sequence?

three nucleotide sequence in mRNA (A,U,G,C)

• code contains 1 start signal and 3 stop signals

• written in units of three letters

• AUGC pairs with UACG

• each triplet code in mRNA is complementary to the anticodon region of its correpsonding tRNA

what are the functions of sigma factors?

bacterial transcirption initiation subunits binding RNA polyermase to form holoenzyme

• allow bacteria to initiate transcription

• control gene expressoin

triplet code of mRNA & complementary sequence

functions of sigma factor

where fMet-tRNA is added/ which site it goes into

tRNAs and how they match codons

wobble hypothesis- what it is + what it allows

alternative splicing on pre-mRNA

what happens to mRNA before becoming mature mRNA (know the list + importance)

inititaion factors (def)

elongation factors (def)

release factors (def)

what affects mutations rate?

loss of function mutations

silent vs nonsense mutations

somatic vs germline mutations

dna repair mechanims & definitions (macthing)

promoter sequences

ribosome binding sites

acetylation changes

demination changes

methylation changes

where does DNA methylation frequently occur?

can a sequence function as an enhancer or silencer ? (explain in detail)

what are the roles of insulators + function of insulators on promoters

what is RNA interference ? + what does it do?

interclating agents and antiseptics

DNA vs RNA vs protein sequences

mutation types

transcription factors

DNA repair mechanisms

comparing somatic vs germline cells (SA)

the steps of mRNA to mature RNA (SA)

role/purpose of insulators (SA)

wobble hypothesis (SA)

gene in red blood cells (is expression high? what is the sequence if treated with DNA DNAse? what is the confirmation? (copy/dose)

what are enzymes role in reverse transcription?

what is the site of gene behavior?