Host Basics_ Microbacterium foliorum

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10 Terms

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Microbacterium foliorum
A strain of bacterium that is widely used as a host for phage isolation in SEA-PHAGES research.
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Actinobacteria
A phylum of gram-positive rod-shaped bacteria that includes the genus Microbacterium.
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Host
The bacterium (in this case, Microbacterium foliorum) used for phage isolation in research experiments.
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Colony
A group of cells derived from a single bacterial cell that grows and forms a visible structure on an agar plate.
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Growth media
The nutrient-rich substance (in this case, PYCa) used to support the growth and division of bacteria.
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Optimal temperature
The temperature range (28-30 ˚C) at which Microbacterium foliorum grows most rapidly.
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Cycloheximide
An antimicrobial compound that can be added to the growth media to prevent the growth of other microorganisms.
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Phage Buffer
A solution containing specific concentrations of Tris, MgSO4, NaCl, and CaCl2, used to maintain the stability of phages during experiments.
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Restriction Enzymes
Enzymes (such as HaeIII, MseI, NspI, SacII, and SalI) used to cut DNA at specific recognition sites, allowing for the manipulation and analysis of DNA molecules.
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Isoschizomers
Different restriction enzymes that recognize the same DNA sequence, which can be used as alternatives to the listed enzymes.