SECONDARY STRUCTURES

Trypsin

cuts off C-terminal side of R or K as long P isn’t C-terminal neighbor

Elastase

endopeptidase that cuts A, G, S, and V on the C-terminal side as long P isn’t C-terminal neighbor

Carboxypeptidase B

exopeptidase that cuts off the C-terminal residue as long as the C-terminal residue is R or K and P isn’t the N-terminal neighbor

Carboxypeptidase A

exopeptidase that cuts off C-terminal residue as long R, K, and P aren’t the C-terminal residue and P isn’t N-terminal neighbor

Cyanogen Bromide

react and cuts on the C-terminal of methionine (M) met

Hydroxylamine

specifically cuts N-Q (Asn-Gln) peptide bonds

Pepsin

endopeptide that cuts N-terminal side of F, W, Y, and L as long P isn’t N-terminal neighbor

Trypsin

endopeptidase that cuts on the C-terminal side of R or K as long P isn’t C-terminal neighbor

Endopeptidase V8

cuts C-terminal side of E (Glutamic acid, 4.07)

Chymotrypsin

endopeptidase that cuts on the C-terminal side of F, W, and Y as long P isn’t C-terminal neighbor

2-Nitro-5-thiocyanobenzoate

cuts on the N-terminal side of C (cysteine) residues

Edman Degradation (Phenylthioscyanite)

Ex: PTC-G

soely ma 1st primary amino group = N-terminal