Cytology and histological correlation

what do epithelial cells look like on cytology and biopsy

- found in - glandular and parenchymal tissues or lining surfaces (mammary gland, liver)

- high cell harvest

- cohesive aggregates

- cell borders distinct - grout lines, tight junctions

• see junctions as white

- polygonal shaped cells, round nuclei

what can cytology/ histopathology tell us

• inflam

• non inflam

what can inflam be

septic

sterile

what can non inflam be

cystic

hyperplastic/ dysplastic

neoplastic

what neoplastic cell types are these

what 3 cell types can neoplastic be

round

mesenchymal

epithelial

What cell type are these

mesenchymal

What cell type are these

epithelial cells

What cell type are these

round cells

what do mesenchymal cells look like on cytology and biopsy

- found in - connective tissue, muscle, bone, cartilage, nerve, endothelial cells

- low cell harvest

- non-cohesive aggregates of individual

- cell borders variable defined, indistinct - wispy

- embedded in matrix

- splindle shaped, cytoplasmic tail

- oval or plump nuclei

what do round cells look like on cytology and biopsy

- 4 diff types

- high cell harvest

- individual cells

- round ish shape

- round or oval nuclei

- look at nuclear:cytoplasmic ration, position of nucleus → distinguish type

what are the 4 types of round cells

lymphocyte, plasma cells, mast cells, histiocyte/ macrophage

Case 1

describe this mass

Cutaneous mass on dorsum

well demarcated, raised, firm, round

Case 1 - Cytology

1. how populated with cells is this image

2. how are cells distributed/ what pattern

3. what is the shape of these cells

4. what cell category

5. what type of cell are they

1. high cell harvest

2. individually

3. round

4. round cell

5. mast cell

why use histopathology after cytopathology confirms cell type?

to grade the tissue and know if metastatic

Case 1 - Histopathology

why would you send this for histo?

highlight mast cell granules - special stain (toluidine blue)

Case 2

describe this mass, what is it likely to be

cutaneous interscapular mass

well demarcated, raised, firm, pale, ulcerated

injection site sarcoma

Case 2 - Cytology

1. what is the cell harvest

2. how are the cells distributed

3. what are the shape of these cells

4. what cell type are they

1. low

2. small cohesive aggregates and individual

3. spindle shaped

4. mesenchymal

is a giant mulinucleated cell on the r

Case 2 histopathology - why would we send this off for histo?

need to send most mesenchymal masses to understand whether they are bening or malignant

Case 3

describe this mass

(cutaneous mass on left next)

well demarcated, firm, round, ulcerated epidermis

Case 3 Cytology

1. what is the cell harvest

2. how are the cells distributed

3. what shape are these cells

4. what cell type are they

1. high

2. cohesive clusters - clear borders

3. polygonal

4. epithelial

Benign trichoblastoma

Case 3 Histopathology

why would you send this ?

location, species, work out if its benign or not