Molbio

This is a technique used in genetic studies that allows the detection and quantification of mRNA

This is a technique used in genetic studies that allows the detection and quantification of mRNA

Reverse Transcription-Polymerase Chain Reaction

RT-PCR refers to the conversion of the __ template into its ____

RNA; COMPLEMENTARY DNA STRAND

This can be sequenced, cloned, and applied to estimate the copy number of specific genes in order to characterize and to validate gene expression

CDNA

These are RNA dependent DNA polymerases which bind to RNA templates and convert them to their complementary DNA sequence in the presence of dNTPs and other essential cofactors

RNA transcriptases

The enzyme reverse transcriptase was first discovered by?

Dr. Howard Temin

Attachment of the virus to a specific receptor on the host cell

Adsorption

Entry of the virus into the host cell

Penetration

It occurs when there is either the separation of the capsid from the genome or rearrangement of the capsid protein exposing the genome for transcription or replication

Uncoating

The eclipse period. The stage when the genetic material is replicated

Synthesis

Genetic material is assembled into a protein coat

Assembly

Viruses are then released from the host cell

Release

In RT-PCR, the RNA template is first converted into a CDNA using a?

Reverse Transcriptase

The ___ is then used as a template for exponential amplification using __

CDNA; PCR

In the one step approach, the entire reaction occurs in a ____

Single tube

In two step reaction, the reverse transcriptase reaction and PCR amplification be performed in ___

Separate tubes

In one step RT PCR, to begin cycling, where are the PCR tubes placed?

Thermal cycler

In one step RT PCR, The first cycle is ___. The second cycle is ___.

Reverse transcription; initial denaturation

True or false. During the second cycle of one step RT PCR, the reverse transcription is activated

False

In one step RT PCR, the next 40-50 cycles are the ___

Amplification program

What are the three steps in amplificatiom program

Denaturation, annealing, elongation

In one step RT PCR, the RT PCR products can then be analyzed with?

Gel electrophoresis