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Anaplerotic reactions
Intermediates in the citric acid cycle can be usedin biosynthetic pathways (removed from cycle). The intermediates need to be replenished to continue
Pyruvate + HCO3 + ATP with enzyme pyruvate carboxylase
oxaloacetate + ADP + Pi
Liver/Kidney
Phosphoenolpyruvate + CO2 + GDP with enzyme PEP carboxylkinase
oxaloacetate + GTP
Heart/Skeletal Muscle
Phosphoenolpyruvate + HCO3 with enzyme PEP carboxylase
oxaloacetate + Pi
Higher plants/yeast/bacteria
Pyruvate + HCO3 + NAD(P)H with enzyme malic enzyme
malate + NAD (p)+
Eukaryotes and bacteria