PAG 1 : Microscopes

stage micrometer function

to calibrate an eyepiece graticule so that it can be used to make measurements

calibrating an eyepiece graticule

select desired objective lens and magnification
line up scales of micrometer and graticule
count number of eyepiece graticule equivalent to each division in the stage micrometer
calculate the length of one division of the stage graticule

procedure for preparing a slide

stain sample with dye
mount sample on slide
place a coverslip on slide, avoiding air bubbles

magnification calculation

size of image / size of object

eyepiece graticule length calculation

1 Eyepiece graticule length = visible stage micrometer length / no. equivalent eyepiece divisions (that fit)