Lab #10: Colony Characteristics and Culture Characteristics

T or F:

The Appearance of Growth is consistent for an organism and is useful to characterize and identify organisms?

True

What are the 7 characteristics of Colony morphology>

1. Shape

2. Margin

3. Elevation

4. Size

5. Color

6. Texture

7. Optical properties

Where do we view to see the shape for colony morphology?

viewed from above

What is the margin for colony morphology?

outer edge line of colony/ parameter of bacterial colony

What/where is the elevation for colony morphology?

side profile

Describe size as a colony characteristic?

Diameter of colonies in mm

Describe color as a colony characteristic?

the color of the colony

Describe texture as a colony characteristic?

the colony surface

Can be Dull/shiny/glossy/wrinkled/Gooey/crusty/dry/powdery

Describe optical properties as a colony characteristic?

how it transmits light

can be: Opaque, translucent, transparent

What terms are used to characterize colony shape?

round, irregular, filamentous, rhizoid

What shape is this? (the small white circles)

round

What shape is this?

Irregular

-lacks a distinct symmetrical shape

What shape is this?

Filamentous

-hair like, fibrous appearance, resembles a mat

What shape is this?

Rhizoid

-rootlike, branched, spreading out in disticnt segments rather then circular form

What terms are used to characterize colony margin?

Smooth, irregular, filamentous, rhizoid, lobate

What terms are used to characterize the elevation?

flat, raised, convex, umbonate, plateau, raised margin

What elevation is this?

Umbonate

What elevation is this? Name in order

raised, flat, convex, umbonate, growth into medium

What elevation is this?

slightly raised but flat

plateau

what optical property is this?

-completely blocks light from passing through, appears solid, colored or white

Opaque

what optical property is this?

-partially transparent, allowing light to pass through but scattering it enough to prevent clear imaging, often resembling frosted glass

Transluscent

what optical property is this?

-Clear, allows light to pass through

Transparent

What culture characteristics in broth is this?

Pellicle

What culture characteristics in broth is this?

ring

What culture characteristics in broth is this?

sediment

What culture characteristics in broth is this?

flocculent

What culture characteristics in broth is this?

turbid

Identify this bacterial growth in an agar plate

-growth resembles a string of beads along the lines of inoculation

Beaded

Identify this bacterial growth in an agar plate

-growth appears toothlike

echinulate

Identify this bacterial growth in an agar plate

-uniform growth along the line of inoculation

Filiform

Identify this bacterial growth in an agar plate

-branched and rootlike appearance

rhizoid

Identify this bacterial growth in an agar plate

-growth spreading away from line of inoculation

Spreading