CLSC 410 Serology Assays

Assays, Detection Method, Reagents, Analyte of Interest, Clinical Utility

RPR Detection Method

Direct Agglutination

(enhanced flocculation)

RPR reagent

Carbon particle cardiolipin antigen

RPR Analyte of interest

Nonspecific treponeme antibody

(REAGIN)

RPR clinical utility

SCREEN for syphilis Ab

VDRL Detection Method

Direct agglutination

(Microscope viewing of flocculation)

VDRL Reagent

cardiolipin-lecithin-cholesterol antigen

VDRL Analyte of Interest

Nonspecific treponeme antibody (REAGIN)

VDRL Clinical Utility

Screen for Syphilis

Can a positive RPR/VDRL confirm infection with syphilis?

NO.

FTA-ABS Detection Method

Indirect immunofluorescent

(non-competitive, signal visible with analyte detection)

FTA-ABS reagent

1. Reiter Ag

2. T. Pallidum detection antigen on slide

3. Fluorescent-tagged anti-antibody

What does Reiter Ag do?

Absorbs out all non-T. Pallidum Abs that might confer a false positive!

FTA-ABS Analyte of Interest

Specific T. Pallidum antibodies

FTA-ABS Clinical Utility

Syphilis Abs CONFIRMATION!

Sure-Vue Mono Detection Method

Direct Agglutination

Sure-Vue Mono Reagent

Horse Erythrocyte

Sure-Vue Mono Analyte of Interest

Heterophile antibody

Sure-Vue Mono Clinical Utility

Screen for IM Heterophile (97% of EBC infections show positive, meaning infectious mono infection for patient)

Rheumatex RF Detection Method

Passive Agglutination

Rheumatex RF Reagent

Latex bound IgG

Rheumatex RF Analyte of Interest

Anti-Antibody (Rheumatoid Factor)

Rheumatex RF Clinical Utility

Evaluation of Arthritis (included in diagnostic criteria)

Sure-Vue Strep A Detection Method

Lateral Flow Immunoassay

(non-comp, signal visible WITH analyte detection)

Sure-Vue Strep A Reagents

1. Anti-strep conjugated antibody pad

2. Test line: anti-strep capture antibodies

3. Control Line: Anti-IgG Ab

Sure-Vue Strep A Analyte of Interest

Strep ANTIGEN from Pt.

Sure-Vue Strep A Clinical Utility

Rapid detection/DIAGNOSIS of Strep throat A

Beta-hCG Detection Method

Latex-Agglutination Inhibition

Beta-hCG Reagents

1. Anti-hCG

   1. hCG coated latex particles

Beta-hCG Analyte of interest

Beta-hCG in urine (ANTIGEN)

Beta-hCG Clinical utility

Determination of pregnancy , or even testicular cancer.

HIV ELISA Detection Method

ELISA

Non Comp (analyte detection with VISIBLE signal)

HIV ELISA Reagents

1. HIV Antigen Adsorbed to well

2. Enzyme linked anti-antibody

   1. Substrate (to cause color change)

HIV ELISA Analyte of Interest

HIV Antibody

HIV ELISA Clinical Utility

Detection of HIV Abs = HIV diagnosis.