LECTURE 2: Types of Pipettes

1. Serologic

2. Mohr

3. Bacteriologic

4. Ball, Kolmer, or Kahn

5. Micropipette

MEASURING OR GRADUATED PIPETTES:

1. Volumetric

2. Ostwald-Folin

3. Van Slyke pipette

4. Sahli’s pipette

5. Pasteur pipettes

6. Automatic micro/macropipettes

TRANSFER PIPETTES:

MEASURING OR GRADUATED

Identify what type (Measuring or Graduated / Transfer) of pipette.

Serologic

MEASURING OR GRADUATED

Identify what type (Measuring or Graduated / Transfer) of pipette.

Mohr

MEASURING OR GRADUATED

Identify what type (Measuring or Graduated / Transfer) of pipette.

Bacteriologic

MEASURING OR GRADUATED

Identify what type (Measuring or Graduated / Transfer) of pipette.

Ball, Kolmer, or Kahn

MEASURING OR GRADUATED

Identify what type (Measuring or Graduated / Transfer) of pipette.

Micropipette

TRANSFER

Identify what type (Measuring or Graduated / Transfer) of pipette.

Volumetric

TRANSFER

Identify what type (Measuring or Graduated / Transfer) of pipette.

Ostwald-Folin

TRANSFER

Identify what type (Measuring or Graduated / Transfer) of pipette.

Van Slyke pipette

TRANSFER

Identify what type (Measuring or Graduated / Transfer) of pipette.

Sahli’s pipette

TRANSFER

Identify what type (Measuring or Graduated / Transfer) of pipette.

Pasteur pipettes

TRANSFER

Identify what type (Measuring or Graduated / Transfer) of pipette.

Automatic micro/macropipettes

SEROLOGIC

TD/blowout pipette.

(TYPE: MEASURING OR GRADUATED)

SEROLOGIC

Has graduation marks to the tip.

(TYPE: MEASURING OR GRADUATED)

SEROLOGIC

Generally, a blow-out pipette.

(TYPE: MEASURING OR GRADUATED)

MOHR

TD/self-draining pipette.

(TYPE: MEASURING OR GRADUATED)

MOHR

Does not have graduations to the tip

(TYPE: MEASURING OR GRADUATED)

MOHR

The tip of the pipette should not be allowed to touch the vessel while the pipette is draining.

(TYPE: MEASURING OR GRADUATED)

MICROPIPETTE

Has a total holding volume of less than 1mL

(TYPE: MEASURING OR GRADUATED)

less than 1mL

Micropipette has a total holding volume of?

MICROPIPETTE

Examples:

• Sahli-Hellige pipette

• Lang-Levey pipette

• RBC and WBC pipettes

• Kirk and Overflow pipette

(TYPE: MEASURING OR GRADUATED)

MICROPIPETTE

It may be designed as either a Mohr or a serologic pipet.

(TYPE: MEASURING OR GRADUATED)

MICROPIPETTE

Measuring pipettes are used to transfer reagents and to make dilutions and can be used to repeatedly transfer a particular solution.

(TYPE: MEASURING OR GRADUATED)

VOLUMETRIC

TD/self-draining pipette

(TYPE: TRANSFER)

VOLUMETRIC

It only measures one volume

(TYPE: TRANSFER)

VOLUMETRIC

Used for non-viscous fluids (like water)

(TYPE: TRANSFER)

VOLUMETRIC

Have been used to add the diluent to a lyophilized control or to measure standards and controls.

(TYPE: TRANSFER)

VOLUMETRIC

This type of pipette usually has the greatest degree of accuracy and precision

TYPE: TRANSFER)

VOLUMETRIC

Should only be used once

(TYPE: TRANSFER)

OSTWALD-FOLIN

TD/blowout pipette

(TYPE: TRANSFER)

OSTWALD-FOLIN

Bulb is closer to the delivery tip, reducing the surface area in contact with the liquid

(TYPE: TRANSFER)

OSTWALD-FOLIN

Two etched continuous rings at the top

(TYPE: TRANSFER)

OSTWALD-FOLIN

Used for viscous fluids

(TYPE: TRANSFER)

OSTWALD-FOLIN

Final drop is blown out

(TYPE: TRANSFER)

VAN SLYKE PIPETTE

Thick-walled capillary tubing with bulb in the center.

(TYPE: TRANSFER)

SAHLI’S PIPETTE

• can deliver 20 ul

• TC

(TYPE: TRANSFER)

20 ul

How much can Sahli’s pipette can deliver?

PASTEUR PIPETTES

Do not have calibration marks and are used to transfer solutions or biologic fluids without consideration of a specific volume.

(TYPE: TRANSFER)

PASTEUR PIPETTES

These pipettes should not be used in any quantitative analytic techniques

(TYPE: TRANSFER)

AUTOMATED MICRO/MACROPIPETTES

• TC

• most routinely used pipette

(TYPE: TRANSFER)

AUTOMATIC MICRO/MACROPIPETTES

measure small amounts of a liquid

(TYPE: TRANSFER)

AUTOMATIC MICRO/MACROPIPETTES

(usually dispense up to 0.1 - 1000 microliter)

(TYPE: TRANSFER)

0.1 - 1000 microliter

How much can automated micro/macropipettes usually dispense?

TO CONTAIN (TC)

Determine the design of this pipette:

Micropipette

TO CONTAIN (TC)

Determine the design of this pipette:

Sahli’s pipette

TO DELIVER (TD)

Determine the design of this pipette:

Serological pipettes

TO DELIVER (TD)

Determine the design of this pipette:

Mohr pipettes

TO DELIVER (TD)

Determine the design of this pipette:

Volumetric pipettes

TO DELIVER (TD)

Determine the design of this pipette:

Ostwald-Folin pipettes

BLOWOUT

Determine the draining characteristic of this pipette:

Serological pipettes

BLOWOUT

Determine the draining characteristic of this pipette:

Ostwald-Folin pipettes

SELF-DRAINING

Determine the draining characteristic of this pipette:

Mohr pipettes

SELF-DRAINING

Determine the draining characteristic of this pipette:

Volumetric pipettes

SELF-DRAINING

Determine the draining characteristic of this pipette:

Van Slyke pipettes