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Which of the following statements concerning beta lactamase is false:

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1

Which of the following statements concerning beta lactamase is false:

Negative test for beta-lactamase always correlates with sensitivity to Gentamicin

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2

The ratio of the amount of antimicrobial that is toxic to the amount, which is effective, is known as:

therapeutic index

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3

The enzyme that breaks penicillin into penicilloic acid is:

Beta lactamase

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4

Antibiotics that act by inhibiting synthesis of the bacterial cell wall include:

Penicillin, methicillin, cephalosporins

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5

A positive beta-lactamase test indicates:

Resistance to penicillin

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6

A procedure, which directly determines beta-lactamase production by a microorganism, is based on the detection of:

Color change resulting from the breaking of beta-lactam ruing of a chromogenic cephalosporin

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7

The initial site of infection with Cryptococcus neoformans is:

Pulmonary

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8

Which of the following organisms is identified by its ability to produce germ tubes in plasma at 37 degrees C in 2 hours:

Candida albicans

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9

A yeast with a capsule seen on an India Ink prep of spinal fluid may be:

Cryptococcus neoformans

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10

Candida albicans is part of the normal flora of which of the following sites?

Throat, vagina, intestinal tract

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11

Which of the following are useful in identifying Candida albicans?

Germ tube positive, urease negative

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12

The most common cause of bacterial meningitis in children 1-4 is:

Haemophilus influenzae

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13

Haemophilus influenzae will grow on:

BAP with a V strip, Chocolate agar

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14

X factor corresponds to which growth factor

Hemin

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15

Haemophilus aegyptius differs most strikingly from Hemophilus influenzae in:

The disease it causes

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16

Hemophilus influenzae:

Grows better on chocolate agar than blood agar

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17

The satellite phenomenon is associated with:

Hemophilus influenzae

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18

The most important cause of meningitis and epiglottis in children, some strains having become resistant to the drug of choice (ampicillin) by means of beta-lactamase enzyme is:

Hemophilus influenzae

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19

You have isolated a gram-negative coccobacillus from an eye culture from a case of conjunctivitis. The organism grows well on chocolate agar but not on blood agar, and further testing show it requires both the X and V factors for growth. The organism is most likely:

H. aegypticus

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20

The porphyrin test is used to identify an organism's requirement for:

Factor x

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21

The routine medium used for isolation of Hemophilus species is:

Chocolate agar

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22

The role of normal skin flora is to:

Compete for attachment sites with pathogens, thus decreasing available sites for pathogens

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23

The collection of organisms referred to as "diptheroids" are:

Normal flora of the skin, gram-positive rods

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24

Which of the following organisms causes furuncles and carbuncles?

S. aureus

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25

Furuncles and carbuncles are abscesses usually caused by:

S. aureus

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26

A specimen from a wound is sent to the laboratory for routine culture. Select the most appropriate method for processing of this specimen.

Direct Gram stain smear, plate on BAP, Choc, and MAC

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27

A satellite test is performed to speciate Hemophilus spp, using BAP. After 24 hours the organism is seen growing around Staph on both BAP. This means the organism requires:

NAD

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28

Hemophilus aegyptius is primarily a pathogen of the:

Eye

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29

Hemophilus influenzae is most commonly encountered as a pathogen in:

Children and elderly

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30

The satellite phenomenon works on the following principles:

Staphylococcus aureus supplies the coenzyme NAD; the blood agar supplies the X factor

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31

Hemophilus parainfluenzae requires which growth factors:

V factor

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32

Normal flora of the skin consists of:

Staph species, alpha-hemolytic Streptococcus sp., Corynebacterium sp.

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33

Skin:

Contains expected flora

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34

The minimal identification information needed to identify a yeast isolate as Candida albicans would be:

Positive germ tube test

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35

A presumptive identification of Cryptococcus neoformans depends on which of the following results?

Negative germ tube, positive urease

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36

The gram stain morphology of the genus Hemophilus is best described as:

Gram negative coccobacilli

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37

Impetigo is most often caused by:

Gp A Strep

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38

Bordet-Gengou media is used for the laboratory diagnosis of:

Whooping cough

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39

The specimen of choice to collect for Bordetella pertussis is:

Nasopharyngeal swab

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40

Pertussis continues to be a problem of worldwide importance. Children under the age of 2 primarily acquire the disease by:

Coming in contact with infected adults who have pertussis

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41

An organism that is a gram-negative coccobacillus, grows on Bordet Gengou agar with mercury drop colony appearance and biochemically inert is:

Bordetella pertussis

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42

A positive cytochrome oxidase test is used to identify:

Neisseria species

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43

Select the best response from the chart given: Glucose - Maltose - Sucrose - Lactose -

Moraxella catarrhalis

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44

Select the best response from the chart given: Glucose + Maltose + Sucrose - Lactose -

Neisseria meningitidis

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45

Both Neisseria meningitidis and Neisseria gonorrhoeae are positive for glucose, but only Neisseria meningitis is positive for:

Maltose

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46

The non-pathogenic Neisseria and Moraxella species have all of the following in common except:

Can cause opportunistic infections of the ear

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47

Neisseria species may be identified by which of the following sugars?

Dextrose, sucrose, maltose, lactose

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48

The Elek toxigenicity test detects the presence of:

Exotoxin C. diptheria

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49

Establishing the pathogenicity of a microorganism isolated from a child's throat as Corynebacterium diptheriae would depend on:

Determining the presence of toxin

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50

The ELEK plate is the in-vitro toxigenicity test for:

Corynebacterium diptheriae

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51

A direct smear from a nasopharyngeal swab stained with Loeffler's methylene blue stain showed various club shaped rods with deep blue metachromatic granules. The likely identification is:

Corynebacterium spp.

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52

Transmission of M. tb is by:

airborne droplet nuclei 1-5

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53

Mycobacterium tuberculosis has:

  • High lipid content in the cell wall

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54

In a normal person, the natural host defenses may ward of M. tb in the lung forming:

Ghon complexes

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55

The Ziehl-Neelson or Kinyouns stain is used for:

Acid fast bacilli

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56

A 51-year-old male was admitted to the hospital for a cardiovascular accident. An aspirated sputum is sent for acid fast studies after several sputa have been negative. The fluorochrome stain shows four acid-fast bacilli, which give a good fluorescence, and is reported to the doctor in charge of TB control. The doctor reports that it does not fit the clinical picture, as the skin test was negative and x-ray normal. A second aspirated sputum was sent. Two fluorescent bacilli are observed. Control slides were used in each situation. With these facts, the most likely conclusion is:

Possible infection with a Mycobacterium spp. other than tuberculosis

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57

The causative organism of tuberculosis is:

Mycobacterium tuberculosis

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58

The small gram-negative rod that causes whooping cough is:

Bordetella pertussis

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59

Specimens must be processed immediately for the isolation of the pathogenic Neisseria species because these organisms are:

Sensitive to temperature extremes and drying

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60

Loeffler's and Cystein-tellurite media are used to isolate the organisms in suspected causes of:

Diptheria

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61

The most characteristic morphological forms of C. diptheriae are found in smears from a 18-24 hours culture on the following medium:

Loefflers medium

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62

Multi-drug resistant tuberculosis results from:

  • Incomplete therapy or non-compliance

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63

The best time to collect a sputum specimen for AFB culture is:

Early morning after 8 hours of fasting

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64

A medium designed for the isolation of Bordetella pertussis is:

Bordet-Gengou

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65

Select the best response from the chart given: Glucose + Maltose + Sucrose - Lactose +

Neisseria lactamica

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66

Which of the following rapid sugars are fermented by the organism Neisseria meningitidis?

Dextrose, maltose

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67

The disease Diptheria is caused by the organism's ability to produce:

Toxin

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68

The toxicity of a strain of Corynebacterium diptheriae is demonstrated by:

Elek plate or PCR

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69

A gram-positive club shaped bacillus occurring in Chinese letter arrangement is probably a member of the genus:

Corynebacterium

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70

The appearance of bright yellow-orange, fluorescent acid-fast bacilli against a dark background is observed after using which of the following stains for Mycobacteria:

Auramine stain

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71

The large lipid content in the cell wall of mycobacterium species is responsible for their:

Acid fastness after staining

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72

A change in colony color upon addition of oxidase reagent is used to identify:

Neisseria species

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73

When a physician orders susceptibility testing on Neisseria meningitidis, what is the fastest method to provide information?

Beta- Lactamase (Cefinase)

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74

A direct smear from a nasopharngeal swab stained with Loeffler's methylene blue stain showed various club shaped rods with deep blue metachromatic granules. The most likely identification is:

Corynebacterium spp

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75

The differentiation between the Neisseria species is made by

carbohydrate fermentation or PCR

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76

The Elek plate is the in vitro toxigenicity test for:

Corynebacterium diphtheriae

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77

All species of Neisseria produce the following enzyme

cytochrome oxidase

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78

Loeffler's and Cystine-tellurite media are used to isolate the organisms in suspected causes of

diphtheria

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79

The enzyme that breaks penicillin into penicilloic acid is`

Beta lactamase

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80

Staph aureus is generally resistant to Penicillin G by what mechanism?

Beta lactamase production

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81

A positive Beta lactamase test indicates

resistance to penicillin

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82

Staphylococcus aureus would most likely be resistant to:

Penicillin

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83

A procedure, which directly determines beta-lactamase production by an microorganism, is based on the detection of

Color change resulting from the breaking of beta-lactam rin of a chromogenic cephalosporin

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84

the test we use to identify candida albicans

germ tube positive

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85

moraxella oxidase result

positive

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86

Acceptable sputum smear

multiple PMNs seen on the smear

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87

rejected sputum smear

if 10 or more squamous epithelial cells are seen per low power field on gram stain

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88

what does beta lactamase tell us

-The enzyme that breaks penicillin into penicilloic acid -Color change resulting from the breaking of beta-lactam rin of a chromogenic cephalosporin -resistance to penicillin -Staph aureus is generally resistant to Penicillin G -When a physician orders susceptibility testing on Neisseria meningitides, the fastest method to provide information

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89

H. influenzae

Chocolate agar with factors V (NAD+) and X (hematin)

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90

H. parainfluenzae

Requires only V factor

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91

Which of the following may be implicated in Neisseria gonorrhoeae infections:

All of the above (ophthalmia neonatorum, urethritis in males, joint and synovial fluid infection [arthritis], pelvic inflammatory disease [PID])

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92

In females, the best site to culture for suspected gonorrhea is the:

Cervix

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93

Select the best response from the chart given: glucose +, maltose -, sucrose -, lactose -

Neisseria gonorrhoeae

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94

The alphabetical symbol used to denote the somatic antigen is the letter:

O

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95

The genus of the family Enterobacteriaceae with which the "swarming" phenomenon is associated is:

Proteus

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96

The medium, which is MOST inhibitory to gram-positive organisms and normal enteric flora, is:

Hektoen

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97

Reactions on a TSI slant were: K/A with gas and H2S. These reactions could be produced by:

Salmonella

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98

A positive urea slant would be typical of which of the following:

Klebsiella pneumoniae

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99

All species of the genus Shigella are characteristically

Nonmotile

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100

Which of the following tests may differentiate Proteus from Providencia and Morganella

H2S

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