Molecular Processing and Protein Based Biological Medicines

These flashcards cover key terms and definitions related to molecular processing and protein-based biological medicines.

Biotherapeutics

Substances created or derived from biological sources which can be used as a drug to treat diseases.

Recombinant Proteins

Proteins that are artificially made by combining genetic material from different organisms.

Monoclonal Antibodies (mAbs)

Antibodies that are identical and produced by one type of immune cell, useful in treating various diseases.

Chinese Hamster Ovary (CHO) Cells

A mammalian cell line widely used in biotechnology for producing therapeutic proteins.

Transfection

Introduction of DNA encoding an antibody into host cells.

Glycosylation

Post-translational modification where sugars are attached to a protein, affecting its stability and effector functions.

Complement Dependent Cytotoxicity (CDC)

Mechanism where the Fc region of an antibody recruits the complement system to lyse target cells.

Antibody Dependent Cellular Cytotoxicity (ADCC)

Mechanism where antibodies mark target cells for destruction by immune effector cells such as NK cells.

Antibody Dependent Cellular Phagocytosis (ADCP)

Mechanism where antibodies enhance the capacity of phagocytic cells to eliminate pathogens.

Upstream Processing

Part of the biomanufacturing process involving manipulation of living cells to produce biological products.

Downstream Processing

Part of the biomanufacturing process involving recovery, purification, and formulation of the biological product.

Cell Expansion

Increasing the number of cells in culture to produce large quantities of an antibody.

Stability

The ability of an antibody to maintain its structure and function over time.

Robustness

The capacity of a cell line to maintain productivity and characteristics over long culture periods.

Post-Translational Modifications

Chemical and structural changes made to proteins after synthesis that are crucial for their function.