plant tissue culture

What is the first step in plant tissue culture?

Selection of explant - choose healthy growing regions from the mother plant.

What nutrients are included in the culture medium?

Micro and macro nutrients, vitamins, sucrose (2-4%), phytohormones for growth, and agar to harden the medium.

What is the pH level of the culture medium?

The pH level is maintained between 5.5 and 5.8.

What is the purpose of sterilization in plant tissue culture?

To eliminate any germs and contaminants from the culture medium or explant.

What is the process for sterilization in plant tissue culture?

Utilize an autoclave machine at 121°C, 15 lb pressure for 20 minutes.

What occurs during callus formation in plant tissue culture?

Callus is formed from the explant under conditions of 3000-5000 lux light, and at a temperature of 17°-20°C with 75% humidity.

What is micropropagation?

Producing a sapling with exact characteristics of the mother plant.

What is meristem culture?

The culture of meristematic tissue to create disease-free new plants.

What is protoplast fusion?

The fusion of protoplasts from two different plants to create hybrid plants.

What is haploid plant creation based on?

It is based on anther and pollen grain culture, specifically androgenic haploid plants.

What is somaclonal variation?

Variation or clones derived from somatic cells during plant tissue culture.

What defines a transgenic plant?

A plant created with desired genes through recombinant DNA processes.

What is the significance of saved embryos in plant tissue culture?

They are hybrids of different species that can produce neutral plants via culture medium.

What is genetic engineering in the context of plant tissue culture?

The process of introducing new characteristics into plants by manipulating genes.