Basic Blood Bank Serology

0.0(0)
studied byStudied by 0 people
GameKnowt Play
learnLearn
examPractice Test
spaced repetitionSpaced Repetition
heart puzzleMatch
flashcardsFlashcards
Card Sorting

1/39

flashcard set

Earn XP

Description and Tags

Fill-in-the-Blank Flashcards

Study Analytics
Name
Mastery
Learn
Test
Matching
Spaced

No study sessions yet.

40 Terms

1
New cards

During routine pre-transfusion testing, and Rh typing must always be performed.

ABO

2
New cards

Weak D testing is _______ for patients, but required for donor red cell components during manufacturing.

NOT required

3
New cards

To detect most clinically significant antibodies, the antibody screen must include incubation at 37 °C and testing at the phase.

antiglobulin (AHG)

4
New cards

The FDA list of clinically significant antibodies includes D, C, E, c, e, M, N, S, s, P1, Lea, Leb, K, k, Fya, Fyb, Jka, and .

Jkb

5
New cards

In tube testing, additives such as LISS, PEG, or enzymes are classified as methods.

enhancement

6
New cards

Solid-phase and column agglutination (gel) are examples of antibody detection systems.

tubeless

7
New cards

A positive autocontrol during antibody identification should prompt performance of a .

Direct Antiglobulin Test (DAT)

8
New cards

The DAT detects in-vivo sensitization of red cells with or complement.

IgG

9
New cards

An _______ spin crossmatch is designed solely to detect ABO incompatibility when no clinically significant antibodies are present.

immediate

10
New cards

When a clinically significant antibody is detected, crossmatched donor units must be antigen and tested through 37 °C and AHG.

negative

11
New cards

Electronic (computer) crossmatch requires two independent determinations of the recipient’s group.

ABO

12
New cards

The minimum statistical requirement for confirming antibody specificity is a probability value of or less.

0.05

13
New cards

Fisher’s Exact Method uses three antigen-positive and three antigen-negative cells, giving a 1 in chance of a random pattern.

20

14
New cards

Elution techniques such as acid or heat are often used to investigate a positive DAT in cases like or hemolytic transfusion reactions.

Hemolytic Disease of the Fetus & Newborn (HDFN)

15
New cards

Warm or cold autoantibodies may require to uncover underlying alloantibodies.

adsorption

16
New cards

Autoadsorption should only be done if the patient has not been transfused within the past months.

3

17
New cards

Front-type ABO discrepancies caused by weak or missing antigens can be resolved by increasing incubation time or using treatment to enhance antigens.

enzyme

18
New cards

Excess plasma proteins causing rouleaux may create apparent additional antigens; the primary corrective step is a replacement.

saline

19
New cards

Back-type discrepancies with weak or missing antibodies are common in newborns because reverse grouping is not required under months of age.

4

20
New cards

A 0.01 M treatment destroys IgM cold-reactive autoantibodies coating patient cells.

dithiothreitol (DTT)

21
New cards

Daratumumab (anti-CD38) therapy can cause pan-reactivity in IAT testing, but red cells treated with eliminate this interference.

DTT

22
New cards

The half-life of prophylactic Rh immune globulin (RhIg) is approximately days.

28

23
New cards

Immediate spin crossmatch or electronic crossmatch may be the sole method only if the patient has no present or historical antibodies.

clinically significant

24
New cards

Antibody identification relies on both positive reactions for pattern recognition and negative reactions to antibodies from consideration.

rule out

25
New cards

A mixed-field reaction in ABO typing often indicates recent transfusion, stem-cell transplant, or .

bone-marrow transplant

26
New cards

Polyagglutination in newborn samples can be caused by contaminating jelly.

Wharton’s

27
New cards

Phenotype or genotype (molecular) typing is particularly useful in multiply transfused patients such as those with disease.

sickle-cell

28
New cards

All discrepancies in donor ABO typing must be resolved before a unit is .

issued for transfusion

29
New cards

Computer crossmatch software must contain logic to alert the user of in data entry or testing.

discrepancies

30
New cards

Rule-out in antibody ID states that if an antigen is present on a non-reactive panel cell, the corresponding antibody is tentatively .

excluded

31
New cards

For front-type weak antigen discrepancies, adsorption/elution or typing can definitively identify subgroups.

molecular

32
New cards

High-incidence antibodies may be suspected when adsorption studies remove all reactivity yet donor cells are still .

incompatible

33
New cards

A newborn’s DAT is used to investigate possible maternal crossing the placenta.

IgG antibodies

34
New cards

In antibody screens, clinically insignificant antibodies should be detected as as possible to avoid delays.

infrequently

35
New cards

Adsorption with selected donor cells is necessary when the patient’s own cells show a population due to recent transfusion.

dual (mixed-field)

36
New cards

Weak D testing at the hospital is not required for confirmatory typing of donor units labeled as Rh .

negative

37
New cards

The presence of anti-B in a 93-year-old A-positive patient’s serum may be detected only after prolonged room-temperature or incubation.

immunoglobulin-enhanced (IAT/extended)

38
New cards

RhIg administration may cause a transient, weakly reactive antibody in pregnant Rh-negative women.

anti-D

39
New cards

Units selected for a patient with Anti-M need only be compatible at /IAT because Anti-M is usually not clinically significant.

37 °C

40
New cards

Patients requiring transfusion who have Anti-Fya and Anti-S should receive red cells negative for and S antigens.

Fya