Lab 9-15 Review: Spectrophotometry, Cell Division, Catalase Assay, Glycolysis & Fermentation

This set of flashcards covers key concepts related to spectrophotometry, binary fission, catalase function, and glycolysis and fermentation as discussed in the lab lectures.

Spectrophotometry

A method to measure the amount of light absorbed or transmitted by a sample as a function of wavelength.

Beer’s Law

States that there is a linear relationship between the concentration of a sample and its absorbance.

Standard Curve

A graph generated by plotting absorbance against known concentrations, used to determine the concentration of unknown samples.

Binary Fission

A form of asexual reproduction in prokaryotic cells, where the cell divides into two identical daughter cells.

Bacterial Growth Curve

A graphical representation of the growth of bacteria over time, including lag, log, stationary, and death phases.

Generation Time

The time it takes for a bacterial population to double in number.

Catalase

An enzyme that catalyzes the decomposition of hydrogen peroxide into water and oxygen, protecting cells from oxidative damage.

Enzymes

Proteins that act as biological catalysts to speed up reactions without being consumed in the process.

Substrate Concentration

The amount of substrate present in a reaction; it affects the rate of product formation in enzyme-catalyzed reactions.

Glycolysis

The metabolic pathway that breaks down glucose to produce pyruvate, generating ATP in both aerobic and anaerobic conditions.