ch. 27-29 btc reproducibility with assays
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6 Terms
what do you need to have to do PCR?
template DNA, primers, DNA polymerase, nucleotides, reaction buffer, and thermocycler
What are the 3 steps of PCR?
denaturation, annealing, and synthesis and repeat
explain what denaturation step is
(92-94 degrees C) separating the double strands in DNA by heating
explain the step of annealing
(37-72 degrees C) it’s when primers bind to complementary sites in DNA (one for the sense strand and one for the non-sense strand)
explain the step of synthesis
(72 degrees C) DNA is copied from template by DNA polymerase and adding bases one at a time.
whats the process of detection
detect on a gel and urn electrophoresis. it can determine size of fragment along with amount by comparing to a DNA ladder with known molecular weights
Note 
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