ch. 27-29 btc reproducibility with assays

what do you need to have to do PCR?

template DNA, primers, DNA polymerase, nucleotides, reaction buffer, and thermocycler

What are the 3 steps of PCR?

denaturation, annealing, and synthesis and repeat

explain what denaturation step is

(92-94 degrees C) separating the double strands in DNA by heating

explain the step of annealing

(37-72 degrees C) it’s when primers bind to complementary sites in DNA (one for the sense strand and one for the non-sense strand)

explain the step of synthesis

(72 degrees C) DNA is copied from template by DNA polymerase and adding bases one at a time.

whats the process of detection

detect on a gel and urn electrophoresis. it can determine size of fragment along with amount by comparing to a DNA ladder with known molecular weights