TOPIC 8.1 - MICROSCOPE'S PARTS/LAB PROCEDURES (REGENTS)

Eyepiece

The part that you look through (closest to the eye) usually 10x

Objective Lens

The magnifying part closest to the slide

(high power = usually 40x; low power = usually 10x)

Fine adjustment knob

Used to focus on low and high power

Course adjustment knob

Used to focus only on low power

Stage

Where the slide is placed

Stage clips

Holds the slide in place

Diaphragm

Controls the amount of light used

How to calculate total magnification:

if a microscope has a 10X eyepiece, and 10X and 40X objectives.
Total Magnification on low power: 10 X 10 = 100X (it looks 100 times bigger than real life)
Total magnification on high power: 10 X 40 = 400X (it looks 400 times bigger than real life)

If you looked at the “E”letter under a microscope, what would it look like?

1. Mirror image and flipped upside down.

2. Increasing magnification reduces the field. (Larger image but you see less of it)

3. Increasing the magnification reduces the amount of light. (Field darkens)