Morphology

S. pyogenes

M protein is a virulence factor of

S.aureus

Protein A is a virulence factor of

M. tuberculosis

Cord factor is a virulence factor of

C. diphtheriae

Babes-Ernst granules

C. tetani

Terminal swollen spores

B. anthracis

central spores

C. botulinum

Subterminal spores

E. coli

#1 cause of community acquired UTI

S. saprophticus

Common cause of UTI in young females and elderly catheterized women

Viridans strep

Most common pathogen and is considered as the Major Throat pathogen

2×3 cm

Size of sputum smear

300 fields

Before reporting a truly negative result , examine at least _______ fields

Acridine Orange

Can be used as counterstain in Fluorochrome method if KMnO4 is not available

Hematoxylin

Fite Faraco’s M. leprae uses what countertain

S. pneumoniae

Lancet or bullet-shaped diplococcus

C. diptheriae

Pleomorphic, pallisades, XYVL formation, chinese letter with club shaped ends (aerobic)

NAL-C

Gold standard for digestion and decontamination of sputum

Gardnerella vaginalis

Clue cells is found on

Clue cells

Squamous with attached bacilli

Fusobacterium nucleatum

Rod with tapered ends, toothpick, spindle-shaped

Borrelia

Loosely coiled spirochete

Vibrio

comma shaped

Leptospira sp

Tightly coiled, one or both ends bent to form hooks

Cutebacterium acnes (vs C. diptheria)

Pallisades, anaerobic diptheroids

B. anthracis

Disjointed bamboo, fishing rod

C. tetani

Terminal, swollen pores, lollipop or drumstick

C. perfringens

Box car morphology

M. leprae

Cigar packet arrangement

M. kansasi

Cross barred, Shepherd’s crook

B. fragilis

Y. enterocolitica

C. granulomatis

P. multocida

Safety pin appearance

Bifidobacterium

bone shaped dog bircuit

Mycobacterium microti

croissant morphology

Camplylobacter

S shaped, wing’s of seagulls with darting motility

H. ducreyi

School of fish

Rail-road track

Finger print appearance

C. clostridioforme

Football shaped

Cardiobacterium hominis

Rosette arrangement

Klebsiella pneumoniae

Develops pink-purple mucoid colonies

Neisseria elongata

Smooth, glistening and clay like

S. aureus

Buttery and creamy yellow

Nocardia

Brittle, Crumby, Wrinkled

Mycoplasma

Fried egg on BAP

Bordetella pertussis

Shiny half pearls on BAP

Mercury droplets colonies

P. aeruginosa

Green metallic sheen on BAP

E. coli

Green metallic sheen on EMB

S. agalactiae

“bull’s eye appearing, gram (+)

M. catarrhalis

Honey puck colonies ( Movable colonies)

M. catarrhalis

Wagon Wheel Appearance

B. anthracis

Beaten egg consistency

B. anthracis

Swirling projections/ Lion head/ Medusa

B. anthracis

Cornet tail appearance

C. diphtheriae

Black colonies with brown halo on Tindsdale

Rhodococcus equis

Salmon pink on BAP

M. tuberculosis

Cauliflower like growth

M. xenopi

Bird’s nest appearing colonies on Corn Meal Agar

Veilonella

Anaerobes showing red fluorescence under UV

Campylobacter Ureolyticus

Pits and corrodes agar

Eikenella corrodens

Pits and corrode agar with typical bleach like odor

S. aureus

Colonies with an odor similar to an old sock on MSA

S. angionosus

Colonies with butter scotch odor

B. anthracis

Inverted fir tree or inverted pine tree on Gelatin Media

C. difficile

Chartreuse on BAP

Barn yard odor

L. monocytogenes

Umbrella like growth

Serratia odorifera

Colonies with dirty, musty odor like potatoes

Serratia rubidea

Red ruby

M. bovis

Waterdroplet appearing colonies

Yersinia enterocolitica

Bull’s eye colonies on CIN

Y. pestis

Hammered copper colonies

Stalactite/ Flocculent growth

B. pseudomallei

Non fermenter, wrinkled colonies with earthy odor

H. influenzae

Shows satellitism

C. septicum

Rhizoid margins appearing like medusa head

(+) swarming

Peptostreptococcus anaerobius

Sweet, fetid odor

Leptotrichia

Raspberry like colonies

Serratia marsescens

Produces prodigiosin ( brick red pigment)

P. aeruginosa

Taco-Corn Tortilla Like/ Overripe grapes

C. violaceum

Ammonium cyanide odor

Produces violet pigment

Nocardia

Fleshly plowed field odor

Legionella

Ground Glass Appearance

T. pallidun

Maintained using testicular chancre of rabbits

S. monoliformis

Breadcrumbs, Puff balls on broth

C. tetani

C. septicum

Swarming motility

Gram (+)

S. pneumoniae

Dome-shaped (24 hrs)

Crater like (48 hrs)

Coin with a raised rim