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Cell Envelope:
All of the surface layers surrounding the cell
Structures that compose the cell envelope may ____ in different types of cells
vary
Cell membranes are ______ included in the cell envelope
always
_____ are amphipathic molecules & are the major component of biological membranes
phospholipids
amphipathic:
Molecule has both hydrophilic and hydrophobic groups
Biological Membrane:
Phospholipid bilayers with other embedded molecules (ex: proteins and cholesterol)
Biological Membrane is also called the _____ membrane or ______ membrane.
cell; plasma
Some biological membranes are comprised of 20-80% ________ by mass that move laterally within the cell membrane
protein
Phospholipids have a _____ head and a _____ tail.
glycerophosphate; fatty acid
Phospholipids have a hydrophilic _____, and a hydrophobic _____
head; tail
Phospholipids use an _____ linkage to connect head group to fatty acid tails
ester
Concentration Gradient:
The difference in the concentration of a substance between 2 areas
A molecule moves _____ its gradient when going from an area of high to low concentration
with (or down)
A molecule moves _____ its gradient when going from an area of low to high concentration
against (or up)
Biological membranes are _____-permeable & act as ______ to prevent diffusion of molecules
semi; barriers
Selectively Permeable
"picky" about what crosses the membrane
Molecules that CAN freely diffuse across membrane without facilitation from a protein
small, uncharged, nonpolar/hydrophobic molecules.
Molecules that CANT freely diffuse across membrane without facilitation from a protein
large, charged, polar/hydrophilic molecules
Types of passive transport
-Osmosis
-Simple (No protein)
-Facilitated (Protein)
Types of active transport
-Primary (Driven by ATP)
-Secondary (Driven by gradient)
Passive transport transports molecules from a
high to a low concentration
Active transport transports molecules from a
low to a high concentration
Osmosis:
passive diffusion of a solvent (usually water) across a semi-permeable membrane
Osmotic Pressure:
helps to maintain shape of cell
Hypotonic solutions have
lower solute concentration
Isotonic solutions have
equal solute concentrations
Hypertonic solutions have
higher solute concentrations
Water will move from
hypotonic to hypertonic solutions
Hypotonic environment causes cell to
swell and potentially lyse (burst)
Hypertonic environment causes cell to
shrink
Bacterial DNA is ______ in shape & found in a region called the _______.
circular; nucleoid
Bacteria have small ________
70s ribosomes
Bacteria divides by ________
binary fission
Cell morphology:
refers to the overall shape of individual cells
cell arrangement:
refers to the organization or alignment of groups of multiple cells
3 Main bacterial cell morphologies
-Coccus
-Bacillus
-Spirillum
Coccus:
Circular shaped bacteria
Bacillus
Rod shaped bacteria
Spirillium
Corkscrew shaped bacteria
Cocci Bacterial Cell arrangements
-Diplococci
-Streptococci
-Tetrad
-Sarcinae
-Staphylococci
Diplococci
2 cells that remain attached
Streptococci
Chain like pattern of multiple cells
Tetrad
Cluster of 4 cells arranged on the same plane
Sarcinae
Cluster of 8 cells arranged into a cube
Staphylococci
Cluster of many cells in an irregular pattern
Bacilli Bacterial Cell Arrangements
-Single bacillus
-Diplobacilli
-Streptobacilli
-Coccobacilli
Single Bacillus
a single cell (most common)
Diplobacilli
2 cells that remain attached
Streptobacilli
chain like pattern of multiple cells
Coccobacilli
short rod or ovals that can appear as diplococci
Spiral Shaped Bacterial Cells
-Vibrio
-Spirillum
-Spirochete
Vibrio
bent shaped short, curved rod
Spirillum
curved rod that forms a spiral
Spirochete
Long spiral with flexible cells
Cilia
Short protein filaments that extend from the cell surface
Ribosomes
Site of protein synthesis in cell cytoplasm
Ribosomes are targets for
antibiotics
Plasmids
Circular double-stranded DNA independent of the chromosome
Inclusions
Molecules vesicles in the cytoplasm
Peptidoglycan
a rigid, mesh-like polysaccharide & protein mix that is the main component of bacterial cell walls
Peptidoglycan is repeating units of 2 different monosaccharides:
N-acetylglucosamine (NAG) and N-acetylmuramic acid (NAM)
Glycan Chain:
sugar chain that is formed by repeating units of NAG and NAM
A 4 amino acid chain is attached to each
NAM molecule, and is important for cell wall structure
Peptide interbridge:
the cross like between tetrapeptides of adjacent glycan chains
Interbridge is only found in
gram-positive cells (tetrapeptides are linked directly in gram-negative cell walls)
Peptide inter bridge forms an interconnected network of
peptidoglycan fibers
Gram-Positive cells stain
purple
Gram-Positive Bacteria cell walls are
thick (small molecules like sugars and amino acids can pass across freely)
Gram-Postitive Bacteria have a gell like substance called the ________ between the cell wall and plasma membrane
periplasm (periplasmic space)
Gram positive bacteria cell walls contain polymers of sugars called
teichoic acids
Teichoid acids:
polymers of glycerol or ribitol connected by negatively charged phosphate groups
Wall teichoic acids:
only linked to the peptidoglycan layer
Lipoteichoic acids:
linked to the cell membrane & spans the entire peptidoglycan layer
Gram-negative cells stain
pink/red
Gram-Negative cell walls are
thin with an outer membrane
Outer membrane:
outer-most layer & is anchored to peptidoglycan via lipoproteins
Lipoprotein:
Protein with a hydrophobic lipid tail that anchors the outer membrane to peptidoglycan
The outer membrane contains
Lipopolysaccharide (LPS)
Lipopolysaccharide (LPS):
large complex molecules with both lipid & carbohydrate components
LPS is also called
endotoxin
LPS (endotoxin) 3 structural parts:
-Lipid A
-Core Polysaccharide
-O Polysaccharide
Lipid A:
Anchors LPS to the lipid bilayer. Released when cell dies, functions as endotoxin
Core Polysaccharide:
Structural molecule connecting lipid A & O polysaccharide
O Polysaccharide:
linked to core polysaccharide; composed of multiple sugars; confers antigenicity (O antigen)
Porins:
permit entry of metabolically important molecules
Porins functions:
provides a barrier to certain antibiotics and other antimicrobial agents; a defense against complement and phagocytosis
Most bacteria are surround by a layer of a sticky gel-like substance called the
glycocalyx
Glycocalyx
Complex polysaccharide layer
Glycocalyx promotes
adhesion of cells to solid surfaces & to other cells
Glycocalyx protects the cell from
dehydration & can collect nutrients from the environment for energy
2 categories of the glycocalyx
-Capsules
-Slime Layers
Capsules:
highly organized & dense layer of polysaccharides tightly anchored to the cell
Slime Layers:
unorganized layer of polysaccharides easily removable from the cell
Glycocalyx is important for the formation of
biofilm
Biofilms:
group of cells encased in a slime-like polysaccharide layer adhered to a surface
Biofilms can be
found on any surface and can cause serious illness
Biofilms are encased by a polysaccharide matrix of
Extracellular Polymeric Substances (EPS)
Extracellular Polymeric Substances (EPS)
Sticky matrix of polymers secreted by cells supporting biofilm structure
Polymers secreted by EPS
polysaccharides, proteins, glycoproteins, glycolipids, and lipids
Pili:
long filamentous protein structures found on the surface of bacterial cells