Bio lab review Gel electrophoresis

Agrose gel electrophoresis technique

A technique used to separate and view fragments of DNA based on molecular size. Not only can demonstrate the presence of DNA but the size as well.

when DNA fragment is placed in chamber, what else is in there with it?

conductive buffer solution

Are DNA particles negatively charged or positively?

negative

Since DNA negative charge what does this mean?

DNA drawn to the positive pole and repelled by negative when placed in electric field. 

Do smaller or bigger DNA Fragments move easier?

smaller. Overtime smaller ones will travel further than larger ones. 

Why is the gel stained?

becaused DNA is colourless if unstained only shows the position of the loading dye not DNA fragments. 

Is the dye positive or negatively charged?

Positive. Therefore bind strongly to DNA fragment.

what is the most common gel type for separating DNA fragments?

Agarose 

what will affect the speed that DNA migrates through gel? (3)

DNA size, concentration of gel, voltage

What is used to cut gel prior to gel electrophoresis? 

Restriction enzymes 

what does electrophoresis not determine? 

sequence determination of fragment 

gel electrophoresis seperate DNA by..

size.

DNA ladder acts as a…

molecular ruler