VTA 120 final exam review

What is used to clean a microscope

Methanol or Alcohol and lens tissue paper

Iris diaphragm

adjusts the size of aperture that allows light to pass through the sample

Nosepiece

holds and turns the objectives

Fecal float test is used to diagnose

Parasite eggs

Fecal smear test is used to diagnose

Protozoa

Performing Fecal float

Mix fecal sample and fecosol

Add fecasol to create meniscus

Place coverslip and wait 10mins

Review slides under microscope

Performing fecal smear

Mix small amount of feces with saline

Apply coverslip and view under microscope

What are different ways to collect urine ?

Free catch, cystocentisis, urinary catheter

What is the most sterile collection method of urine

Cystocentesis

What test are preformed with urine

Specific gravity, strip and sediment-stain

What is a refractometer

Measures dissolved solids in liquid

What test are done with a refractometer

Specific gravity total protein

Antigen

chemical substance that triggers an immune response by the body. ( Vaccine or pathogen)

ELISA test

Identify antigens

Enzyme linked immuno sorbent assayz

What are laboratory books

used to track all tests that have been run in house or sent to a reference lab

Performing Ear cytology

Test both ears

Swab in ear

Roll thin layer on slide

Heat fix and stain

View on all magnifications and 100x with Emerson oil

Performing skin scraping test

Apply mineral oil to skin using a blade

scrape off layers of skin

Spread on microscope slide with cover slip

DTM

Dermatophyte test media

Differentiate fungus by change in color

Hair is blocked from site

What is proper PPE for DTM and

Látex gloves

What is proper PPE for necropsy

Gloves

Plastic apron

Goggles

Necropsy

An autopsy