MLS AMT Definitions

Molecular instrumentation: STR

Molecular instrumentation: Widely used for DNA sequence

Molecular instrumentation: FISH

Molecular instrumentation: Localization of a specific DNA sequence or an entire chromosome in a cell. Detects targets in cytology/histology

Sothern Blot

Detects specific DNA sequences by restricting endonuclease and uses gel electrophoresis

Western Blot

Detects and identifies proteins within a sample using gel electrophoresis

Apolipoproteins

Proteins on the surDATface of lipoproteins. Like most proteins, they can be detected/measured using antibodies

DAT

Used to detect antibodies sensitizing RBCs in vivoIA

IAT

Detects antibodies in plasma

Sensitization

The iniCoatitial binding of and antibody to antigen on RBCs surface. 1st step in the immune process

Coating

The result of binding where the RBCs become covered with antibodies

B cell lymphocytes

Type of lymph responsible for producing antibodies

Helper T Cells (CD4+)

Type of lymph that coordinates immune response and directly attacks/kills infected cells and tumor cells. They produce cytokines. Adaptive immune response

NK Cells

Type of lymph that attacks/kills infected cells and tumor cells but without the need for prior sensitization. Innate immune response

Sensitivity +

The degree in which a test can identify a + result for a disease

Specificity -

The degree in which those without the disease will test negative

Indirect (Unconjugated) Bilirubin

0.0-0.3 mg/dL

Produced by the breakdown of RBCs and binds to albumin for transport to the liver. Fat soluble (insoluble)

Direct (Conjugated) Bilirubin

0.0-0.7 mg/dL

Formed in the liver, water soluble, and converted to urobilinogen (excreted in bile 18%, stool 80% and urine 2%)

CD45 marker

A marker present on lymphs, monos, and granulocytes - immune cell signaling

(not platelets and erythrocytes)

1. Reticulocyte 2. HgH Bodies

1. Immature RBCs with darker inclusions using supravital stain

   1. Faint blue inclusions resembling sugar using supravital stain

Amphetamine perscription

Prescribed for narcolepsy and ADHD

Digoxin prescription

Prescribed for congestive heart failure

Dilantin prescription

Prescribed for treatment of therapeutic seizure disorders

Phenobarbital prescription

A slow acting barbituate that controls seizures