Histo Sem W2: Clearing

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15 Terms

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clearing

removal of dehydrating agent, replaced with this, giving tissue a “translucent (transparent) appearance“

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high refractive indices

translucent/transparent is because of

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xylene

most common

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xylene

most rapid

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toluene

may be used as substitute for xylene and benzene

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benzene

rapid-acting

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benzene

for urgent biopsies

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chloroform

for large and tough tissue specimens

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chloroform

does not become translucent

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carbon tetrachloride

similar to chloroform

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carbon tetrachloride

relatively cheaper

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cedarwood oil

for CNS, cytological studies

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aniline oil

embryos, insects, and very delicate specimens

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clove oil

tendency to become adulterated

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methyl benzoate & methyl salicylate

double embedding techniques