molecular midterm 2 assays

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9 Terms

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what does northern blot do

measure RNA abundance

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nuclear run-on assays

measure transcription rate

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reverse-transcription PCR

detect and quantify RNA by converting it into cDNA

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Why is RT-PCR useful?

Detects low levels of mRNA or viral RNA

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S1 mapping

determine the precise 5' or 3' ends of an RNA transcript; identify transcription start and stop sites

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primer extension

maps the precise 5' end of RNA

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EMSA

detects protein-DNA binding

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DNase footprinting

Identifies where proteins bind on DNA

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Promoter+Reporter gene assays

Measures promoter activity by linking it to a reporter gene