molecular midterm 2 assays
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Last updated 8:24 AM on 2/27/25
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9 Terms
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what does northern blot do
measure RNA abundance
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nuclear run-on assays
measure transcription rate
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reverse-transcription PCR
detect and quantify RNA by converting it into cDNA
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Why is RT-PCR useful?
Detects low levels of mRNA or viral RNA
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S1 mapping
determine the precise 5' or 3' ends of an RNA transcript; identify transcription start and stop sites
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primer extension
maps the precise 5' end of RNA
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EMSA
detects protein-DNA binding
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DNase footprinting
Identifies where proteins bind on DNA
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Promoter+Reporter gene assays
Measures promoter activity by linking it to a reporter gene
Note 
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