Microbiology Lab Practical 1

Know the parts of the microscope

Know the microscope parts with their functions

400x

Bacillus

True

Coccus

Spirillum

False

False

Label the bacterial cell model to review prokaryotic cell parts.

Coccus

Bacillus

Spirillum

Tetrad

Cocci in chains

False

Memorize

True

b. Make smear-->air dry-->heat fix

Negative Staining

b. Acid alcohol

b. Acid fast; non-acid fast

True

e. Both a and b are correct

a. Both the bacterial cell wall and the dye are negatively charged, so they repel each other

Know parts of Capsule

c. Peritrichous

False

False

True

Tube 1

d. 0.3%-0.5%

Know the terms about flagella and motility

b. Two

c. Streak plate method

d. Color, elevation, texture and margins

e. Both 3 and 4

b. Each colony will get bigger until they run out of space and nutrient

False

b. All cells are descedents of the same bacteria.

b. Salt intolerant

d. Detect hemolysis and provide nutrients for growth of fastedious bacteria

b. Beta hemolysis

Recognize Growth or No Growth

b. Presence of acid and gas

d. Bromcresol purple

False

True

False

b. At the bottom

Know Gas Requirements definitions

c. E.coli B

True

a. The bactriophage is specific to the host.

True

b. the bacteriophage is undergoing lytic cycle

b. Over the lawn of a bacteria

c. Zone of inhibition

False

c. Tetracyclin

a. Penicillin

c. Millimeter

d. Broad spectrum

Know Staining dyes