gluconeogenesis reaction locations

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Where does the first regulatory checkpoint of gluconeogenesis occur?

In the mitochondria (pyruvate carboxylase converts pyruvate to oxaloacetate)

2
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Where does the second regulatory checkpoint of gluconeogenesis occur?

In the cytoplasm (phosphoenolpyruvate carboxykinase converts oxaloacetate to phosphoenolpyruvate)

3
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Where does the third regulatory checkpoint of gluconeogenesis occur?

In the cytoplasm (fructose 1,6-bisphosphatase converts fructose 1,6-bisphosphate to fructose 6-phosphate)

4
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Where does the dephosphorylation of glucose 6-phosphate occur?

In the endoplasmic reticulum/liver (catalyzed by glucose 6-phosphatase, which is primarily in the liver