SWEAT

Sweat

A liquid made from 99% water and 1% salt and fat

Water

99% in sweat

Salt and Fat

1% in sweat

Cystic Fibrosis

Autosomal recessive metabolic disorder affecting the mucous secreting glands of the body

770 mEq/L

Reference Value for Cystic Fibrosis Diagnosis

Principle

Pilocarpine (a cholinergic agonist) stimulates sweat production when introduced into the skin using a mild electric current (iontophoresis) of 0.15 mAcurrent for 45 minutes

Gene Involved

(MUTATION) Cystic Fibrosis Transmembrane Conductance Regulator on chromosome 7

• Pancreatic Insufficiency

• Respiratory Distress

• Intestinal Obstruction

Associated conditions in Cystic Fibrosis

Gibson & Cooke Pilocarpine Iontophoresis Test (Sweat Chloride Test)

Primary diagnostic test for Cystic Fibrosis

Absorbent pads/ Macroduct coil system

Sweat Sample Collection

Measurement

Chloride concentration in sweat is analyzed by titration, ion-selective electrode, or coulometric methods.

Pilocarpine

A cholinergic agonist

Defective Chloride channel

Thick, sticky secretions affecting lungs, pancreas, and intestines.

Iontophoresis

stimulates sweat production when introduced into the skin using a mild electric current