Transmission Electron Microscopy (TEM)

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5 Terms

1
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What are 2 difference with light microscopy?

- Shorter wave length = high resolution
- Uses e- instead of light photons

2
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How are electrons used? (2)

- e- beams are focused by electromagnetic lenses in vacuum
- e- passes specimen and gets excited so emits light

3
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Magnification ratio?

1000 - 250,000

4
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How is an image made?

- Due to bouncing off excited electrons = emits light

5
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Why does specimen need to be fixed and stained with HEAVY METALS? (2)

- Cells have LOW ATOMIC NUMBER so SCATTERING is poor
- Increase density = Increase contrast