Transmission Electron Microscopy (TEM)
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Last updated 1:42 PM on 2/4/24
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5 Terms
1
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What are 2 difference with light microscopy?
- Shorter wave length = high resolution
- Uses e- instead of light photons
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How are electrons used? (2)
- e- beams are focused by electromagnetic lenses in vacuum
- e- passes specimen and gets excited so emits light
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Magnification ratio?
1000 - 250,000
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How is an image made?
- Due to bouncing off excited electrons = emits light
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Why does specimen need to be fixed and stained with HEAVY METALS? (2)
- Cells have LOW ATOMIC NUMBER so SCATTERING is poor
- Increase density = Increase contrast
Note 
Flashcards (45)