Cell and Gene Therapy Bioreactors Week 9

Cell therapy Biomanufacturing

ell therapy is usually patient specific and involves the use of primary cells from patients. Unlike CHO cells, primary cells can’t be grown forever. (Non-cancerous cells age just like the rest of us.) You don’t need to make 20,000 L batches. You typically make 2-3 L batches.Therefore bioreactor designs other than stirred tank are typically used in cell and gene therapy manufacturing.

How a Chimeric Antigen Receptor (CAR) T-cell works

Steps involved in typical CAR-T Therapy

1. Leukapheresis, the patients T cells are separated from the rest of the blood.

2. Modification of T lymphocytes,  The CAR construct is inserted into the T cell with use of non-viral transduction, and the CAR construct is inserted into the T cell with the use of viral transduction.

3. Administration of CAR-T Cells to the patient

Lymphocytes

a vital type of white blood cell that forms a crucial part of the body's immune system, with three main types: B cells, T cells, and natural killer (NK) cells. B cells create antibodies to neutralize pathogens, T cells destroy infected or cancerous cells and regulate the immune response, and NK cells kill compromised cells. They are produced in the bone marrow and are found in the blood, lymph, and lymphoid tissues like the lymph nodes and spleen, helping to fight infections, cancer, and foreign bodies