Biochem Ch 3.1: Amino Acids

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Last updated 12:49 AM on 2/12/26
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23 Terms

1
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What are the subclasses of amino acids?

  1. Non-polar aliphatic

  2. Polar, Uncharged

  3. Positively Charged

  4. Negatively Charged

  5. Aromatic

2
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Every amino acid is chiral at the alpha C except for…

Glycine

3
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99% of amino acids exist in what form?

L, NOT D form!

4
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What are the non-polar aliphatic amino acids typically used for?

  • Directing/stabilizing protein-folding and internal structure

  • The hydrophobic effect

5
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Which aromatic amino acid absorbs UV light the best?

  • Tryptophan!!

  • Next is tyrosine, then phenylalanine

6
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Which amino acid is known for forming disulfide bridges with it’s other amino acids?

Cysteine

7
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Attributes of polar, uncharged amino acids

  • Soluble in water/aqueous environment

  • Hydrophilic

  • Form H-bonds with water

  • On protein surfaces

8
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What is the dimeric amino acid called when two cysteines form a disulfide bond?

Cystine!

9
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The net charge of the positively or negatively charged amino acids depends on…

the pH of the solution

10
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What are positively charged amino acids typically used for?

Ionic interactions, for example with nucleic acids

11
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What are negatively charged amino acids typically used in?

Electrostatic interactions with positively charged amino acids

12
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What is the electrostatic interaction between a positive and negative amino acid called?

A salt bridge

13
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We write amino acids in what direction?

N terminus to C terminus

14
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When pH«pKa, the amino acid is…

Fully protonated

15
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When pH»pKa, the amino acid is…

Deprotonated

16
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At what point in a titration does pH=pKa?

At the midpoints (half way to the inflection points)

17
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At neutral pH, amino acids exist in what state?

zwitterion state

18
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What is the isoelectric point (pI)?

pH when the amino acid has a 0 net charge

19
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For amino acids with no ionizable group other than their side chain, how do you find their pI?

it is the inflection point on a titration.

20
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What is the pKa range for the carboxyl group on an amino acid side chain?

1.8-2.4

21
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What is the pKa range for the amino group on an amino acid side chain?

8.8-11.0

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amino acids are connected via…

amide linkages

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Average mass of an amino acid =

110 Da