BI 157L: Chlorophyll Study Guide

light condition, soil type, and seed type

What are the controlled variables?

follow a lab procedure to extract the chlorophyll from the plant leaves and then analyze the images with image J to measure the percent greenness

How will we achieve the objective of this experiment?

To analyze the percent greenness of two different seed types, in two different soil types, and placed in varying conditions

What is the objective of this experiment?

The amount of chlorophyll produced

What does the % greenness indicate?

Image J by splitting the picture

How will we analyze the % greenness in the photos?

Cowpeas and corn seeds

What are the different types of seeds being used?

Cowpeas are legumes and eudicots

What are cowpeas?

Corn are part of the grain family and considered monocots

What is corn?

Lawn soil and potting soil with Miracle Grow

What are the two soil types?

Inside the thylakoids of the chloroplast

Where can we find chlorophyll?

Light conditions have a crucial role in chlorophyll production which impacts the growth, development, and overall health of plants

Does light condition impact the amount of chlorophyll produced?

Fertilizer does impact the amount of chlorophyll produced because they provide important nutrients like nitrogen, phosphorous, potassium, magnesium and iron BUT excessive amounts of fertilizer is unhealthy

Does the presence or absence of fertilizer impact the amount of chlorophyll produced?

soil types and light conditions are important in plant growth because it is an ideal condition for healthy plants

How do these different conditions and treatments impact leaf growth? How does it impact the presence of chlorophyll?

Soil microbes are important in leaf pigmentation because they are in charge of plant health

Do soil microbes play a role in leaf pigmentation?

The natural compound present in green plants which gives them the color

Chlorophyll

• 30 grams of assigned soil

• 2 cups

• 6 assigned seeds

• water

What are the materials needed?

The darker the more green

What does it mean when the pixel is darker?

The closer to 0 the more black

What does it mean when the pixel is closer to 0?

The closer to 255 the more white

What does it mean when the pixel is closer to 255?

the darker a pixel the more green

What does it mean when the pixel is darker?

The closer it is to 0, the more green

What does it mean when the pixel is closer to 0 for greenness?

10 mL

How many mL of water was added to the cups?

30 g

How many grams of soil was added to the cup?

• mortar and pestle

• graduated cylinder

• 95% ethanol

• ceramic wells

• phone timer & camera

What materials are needed for chlorophyll extraction?

5 mL

How many mL of ethanol was added to the well?

Red and Blue

What light colors does the plant absorb?

Green

What light color does the plant reflect?

0-255

What is the pixel range of the images?

mean/255 × 100

How do you find the percent greenness?