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Magnification
How much bigger an object appears COMPARED to its actual size
Resolution
How close 2 separate entities appear
Name 2 uses of staining
1) Makes organelles visible
2) Can distinguish organelles
3 differences between Light Microscope and Electron Microscopes
1) You can see colour and movement under light microscope
2) Electron microscopes have a higher resolution
3) Electron microscopes have a higher magnification
Formula for Magnification
Magnification = Image/ Actual size
Magnification and Resolution for ELECTRON MICROSCOPES
Magnification - 1 million x
Resolution - Less than 1nm
Magnification and Resolution for LIGHT microscope
Magnification - 400x
Resolution - 200nm (speed of light)
2 types of ELECTRON microscopes & differences
1) Electron Transmitting Microscope -
Micrographs are 2D
Not in colour
2) Electron Scanning Microscope
3D
Not in colour