Microscopes

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Biology A-Level

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8 Terms

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Magnification

How much bigger an object appears COMPARED to its actual size

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Resolution

How close 2 separate entities appear

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Name 2 uses of staining

1) Makes organelles visible

2) Can distinguish organelles

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3 differences between Light Microscope and Electron Microscopes

1) You can see colour and movement under light microscope

2) Electron microscopes have a higher resolution

3) Electron microscopes have a higher magnification

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Formula for Magnification

Magnification = Image/ Actual size

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Magnification and Resolution for ELECTRON MICROSCOPES

Magnification - 1 million x

Resolution - Less than 1nm

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Magnification and Resolution for LIGHT microscope

Magnification - 400x

Resolution - 200nm (speed of light)

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2 types of ELECTRON microscopes & differences

1) Electron Transmitting Microscope -

  • Micrographs are 2D

  • Not in colour

2) Electron Scanning Microscope

  • 3D

  • Not in colour