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autotroph
can use inorganic sources of carbon to make it into food
heterotroph
obtain carbon from other sources (organisms)
essential nutrient
carbon, hydrogen, oxygen, nitrogen, phosphourus, sulfur
macronutrient
required in large amounts
cell structure, metabolism
ex.) C, H, O
saprobe
a microbe that decomposes organic remains from dead organisms
osmosis
diffusion of water through a selective membrane
diffusion
the dispersal of molecules down a concentration gradient by random moion to achieve a uniform distribution
isotonic
2 solutions havng the same osmotic pressure and having no net movement of solvent
hypertonic
having a greater osmotic pressure than a reference solution
hypotonic
having a lower osmotic pressure than a reference solution
active transport
movement of molecules from low to high AGAINST the concentration gradient
USES ENERGY
facilitated diffusion
movement of molecules from high to low with the use of PROTEINS
DOES NOT USE ENERGY
endocytoisis
type of active transport
movement of molecules into the cell via invagination
exocytosis
also active transport
pinocytosis
engulfing of liquids (oils, mols.)
phagocytosis
engulfing of whole cells (amoebas, wbcs)
psychrophile
found in cold water, soil, not in humans
psychrotolerant
grow slowly at low temperature, can cause food to spoil
mesophile
most medically significant bacteria; human pathogens
can also be psychotolerant and thermoduric
thermophile
hot springs
extreme thermophile
archaea, deep-sea vents, require autoclaving (heating in autoclave) for isolation
optimum temperature
intermediate range that promotes fastest growth rate and metabolism
thermoduric microbe
survives small expoures to high heat, contaminate pasteurized foods (giardia, bacillus, clostridium)
obligate aerobe
must have oxygen to live, have enzymes to process oxygen (fungi, protists, bacteria)
obligate anaerobe
can not grow if oxygen is present, do not have enzymes to process oxygen
microaerophile
do not grow at normal oxygen concentrations, require a little amount of oxygen
facultaive anaerobe
can grow in aerobic conditions but can switch to anaerobic metabolism whn there is no oxygen, have enzymes to process oxygen
aerotolerant anaerobes
do not use oxygen but can survive in the presence of oxygen (have alternatemethods to break peroxides and superoxide)
halophiles
can grow best at high salt concentrations
barophiles
loves pressure (keeps enzymes in correct shape), die at standard atmosphere pressure
acidophiles
loves acidic pH (1.0-5.5)
alkhaliphiles
loves basic pH (8.5-11.5)
neutrophiles
loves neutral pH (most bacteria)
pH 5.0-8.0
used in preservation, pickling (inhibits growth)
lag phase
cells are adjusting to media, synthesizing new material needed for replication
exponential growth phase
(log phase)
cells produce primary metabolites (compounds needed for growth, waste)
stationary phase
cells are dying, release molecules that other cells can use for growth
death phase
end of cell growth due to lack of nutrition, depletion of environment, and accumulation of wastes
chemostat
device that supplies fresh medium to the growth chamber, withdraws old medium to maintain logarithmc growth
turbidity
cloudy appearance of nutrient solution in a test tube due to growth of microbe population