Exercise 2 - Structures and Composition of Microorganisms

VIRULENCE

ability of the microorganism to cause infection/disease to a particular host like humans

VIRULENCE MECHANISM

how that particular bacteria structure participates/enables the organism to cause infection/disease to any human host

• GRANULAR INCLUSION

• BODIES/METACHROMATIC GRANULES

They are otherwise known as polar bodies

GRANULAR INCLUSION

They are irregularly-shaped circles/irregularly-shaped spheres (circular structures) present within the body of the organism

GRANULAR

Looking at irregularly shaped circles

INCLUSION

Irregularly shaped circles/granules are found inside the body of the organism

METACHROMASIA

They are known as metachromatic granules because it exhibits a special property called

METACHROMASIA

a special staining property of granular inclusion bodies where it appears different color from the color of the staining used to demonstrate it

METHYLENE BLUE

PRIMARY STAIN

• MONOTRICHOUS

• LOPHOTRICHOUS

• AMPHITRICHOUS

• PERITRICHOUS

MESSEA’S CLASSIFICATION

• NUMBER OF FLAGELLA

• LOCATION OF FLAGELLA

MESSEA’S Classification. It is classified according to two different criteria:

MONOTRICHOUS

# of flagella: one flagellum

location: on one side or either side of the organism

LOPHOTRICHOUS

# of flagella: tuft (bundle) of flagella

location: on one side of either side of the organism

AMPHITRICHOUS

# of flagella: one flagellum

location: both sides of the organism.

= 2 flagella on both sides

PERITRICHOUS

# of flagella: abundant number of flagella

location: scattered all throughout the body of the organism

ATRICHOUS

• no flagella

SLIME LAYER

very thin, almost not apparent in the microscopic image

CAPSULE

very defined, apparent, and conspicuous polysaccharides surrounding the microorganism is bigger and more visible

CENTRAL ENDOSPORE

spore in the center of the body of the organism

SUBTERMINAL ENDOSPORE

spores near the end of the body of the organism

TERMINAL ENDOSPORE

spore at the end of one or both sides of the body of the organism

TERMINAL ENDOSPORE

cotton bud/lollipop-shaped/drumstick appearance

• METAPHOSPHATES

• POLYPHOSPHATES

GRANULAR INCLUSION BODIES/METACHROMATIC GRANULES

COMPOSITION:

FLAGELLIN

FLAGELLA

COMPOSITION:

CALCIUM DIPICOLINATES

ENDOSPORES

COMPOSITION:

POLYSACCHARIDE

SLIME LAYER

COMPOSITION:

POLYSACCHARIDE

CAPSULE

COMPOSITION:

ENERGY RESERVE

GRANULAR INCLUSION BODIES/METACHROMATIC GRANULES

VIRULENCE MECHANISM:

MOTILITY AND ANTIGENIC PROPERTY

FLAGELLA

VIRULENCE MECHANISM:

RESISTS DISINFECTION

ENDOSPORES

VIRULENCE MECHANISM:

ATTACHMENT MECHANISM

SLIME LAYER

VIRULENCE MECHANISM:

ANTI-PHAGOCYTOSIS & ANTI-COMPLIMENTARY

CAPSULE

VIRULENCE MECHANISM:

CORYNEBACTERIUM DIPHTHERIAE

GRANULAR INCLUSION BODIES/METACHROMATIC GRANULES

REPRESENTATIVE ORGANISM: (Babes Ernst granules)

MYCOBACTERIUM TUBERCULOSIS

GRANULAR INCLUSION BODIES/METACHROMATIC GRANULES

REPRESENTATIVE ORGANISM: (Much granules)

PSEUDOMONAS AERUGINOSA

FLAGELLA

REPRESENTATIVE ORGANISM: MONOTRICHOUS

BARTONELLA BACILLIFORMIS

FLAGELLA

REPRESENTATIVE ORGANISM: LOPHOTRICHOUS

SPIRILLUM SERPENS

FLAGELLA

REPRESENTATIVE ORGANISM: AMPHITRICHOUS

• SALMONELLA TYPHI

• PROTEUS VULGARIS

FLAGELLA

REPRESENTATIVE ORGANISM: PERITRICHOUS

BACILLUS SUBTILIS

ENDOSPORES

REPRESENTATIVE ORGANISM: CENTRAL / SUB-TERMINAL

CLOSTRIDIUM TETANI

ENDOSPORES

REPRESENTATIVE ORGANISM: TERMINAL

SARCINA LUTEA

SLIME LAYER

REPRESENTATIVE ORGANISM:

• STREPTOCOCCUS PNEUMONIAE

• KLEBSIELLA PNEUMONIAE

CAPSULE

REPRESENTATIVE ORGANISM:

MYCOBACTERIUM TUBERCULOSIS

CORYNEBACTERIUM DIPHTHERIAE

PSEUDOMONAS AERUGINOSA

BACILLUS SUBTILIS

CLOSTRIDIUM TETANI

SARCINA LUTEA

KLEBSIELLA PNEUMONIAE