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Prior to harvest or sitting after colcemid

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Addition of 1st fix

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After 1st spin

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2nd fix and spun

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Spin after pre-fix

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Post hypo

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Invert after pre-fix

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After centrifuge, pre Hypo

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Inversion of 1st fix

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After wash, EB, and colcemid

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3rd fix and spun

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Addition of pre-fix