microscope

what does the light microscope use

uses light to magnify objects up to 1,500x actual size

light microscope resolution

low- have an resolution up to 0.2 μm- not large enough to see smaller organelles

what is the max magnification of a light microscope

X1500

what are light microscope used for visualising

whole cells or tissues

what is an advantage of a light microscope

can watch living cell we cant see processes such as cell division irl

what do electron microscope use

instead of light it uses electrons

electron microscope resolution

higher giving more detailed images

2 types of electrons microscope

1. transmission electron microscope

2. scanning electron microscope

what do transmission electron microscope use

electromagnets to focus a beam of electrons which is than transmitted through the specimen

which part of the specimen absorb more electrons

the denser part making them more darker on the image i end up with

resolution of transmission electron microscope

higher- around 0.0002 μm allowing us to see individual organelles

why do transmission electron microscope have a higher resolution then light

Electrons have a much shorter wavelength compared to visible light

limitation of transmission electron microscope

only can be used on thin specimens and cant see live cells

max magnification

1,000,000 X

what scanning electron microscope do

scan a beam of electrons across the specimen knocking of electron from the specimen which is used to create an image

scanning electron microscope resolution

lower resolution then TEM having a max of 0.002 μm

scanning electron microscope image

is 3D and colourless and cant be used for live cells

max magnification scanning electron microscope

500,000 x

Vacuum EM

cells are in an Vacuum meaning they are dead