Generation of ATP

Where is glycolysis located

IN the cytosol

What are the inhibitors of PFK 1? How do they make logistical sense?

ATP and citrate since there’s are down stream products which tell PFK 1 that we are in a high energy state already.

What is PK activated by and why

It is inhibited by ATP and citrate,

Activated by AMP and F26BP

What is private kinase activated and inhibited above

They are inhibited by upstream F16BP

Inhibited ATP

Where is ATP used and produced in glycolysis

ATP is being used by PFK1 and ATP is produced by private kinase

Ie net of 2 ATP from 4 made- 2 used.

When insulin is high what is activated?

Activated both PFK1 and pruvate kinase

**** insulin to glucagon levels regulate key enzymes ie glucokinase, PFK1 and pyruvate kinase

When glucagon is high what happens

Inh what insulin activates, ie oppsoite effect

How is PFK1 regulated int he liver?

PFK2 produces F26BP which activates PFK1.

• High insulin + increased F26BP than more active PFK1.

• PFK2 adds phosphate to F6P to activate it to ???

How is glucose transported into the cell

What are the syntoms of GLUT 1 deficiency

Inherited disorder, intractable seizures, dev delay, no medication can fix this.

What is the only insulin depend transporter

GLUT4 - where? Obviously the muscle and adipose tissue.

What is the transporter which transports fructose and where is itfound

GLUT5- small intestine and testes

Is hexokinase or glucokinase specific?

hexokinase is non specific and glucokinase only works on glucose.

What does arsenic affect

The binding of phosphate to Glyceraldehyde 3 phosphate

ldolase deficency

Frites intolerance or aldolase deficency vs fructose kinase deficiency or essential frutosuria

Where is ladolase b vs a located

Ladolase B is in the LIVER.

A patient is found with no mitochondria, what will they have to resort to in terms of energy?

They will have to use glycolysis as their primary source of anaerobic repsitoation as even though they may have oxygen they will not have the mitochondria to go through the TCA cycle.

What kind of phosphorilization does glycolysis go through compared to the TCA?

They go through substrate level phsoprylization compared to oxidiaitive phosphorilization.