Pre-Lab Safety pt 1

When should you disinfect your workbench?

Before and after lab work.

Which of the following actions is encouraged in the laboratory?

Placing items you won't be using during lab time in designated areas.

Which of the following actions should you AVOID in the laboratory?

Placing culture tubes on their sides.

Food and drinks are not allowed in the laboratory primarily because they may __________.

transfer microbes into your body

What should you do if you need to leave the laboratory temporarily in the middle of your work?

Remove your lab coat and gloves.

Why should you decontaminate your work area prior to beginning lab work?

To prevent the potential transfer of microbes to you and your experiment.

While removing a clean glass culture tube from its box, you accidentally drop the tube, and it shatters on the floor. Which of the following best describes what should you do?

Sweep up the broken glass, and dispose of it in a designated container for broken glass.

You knock over a glass culture tube containing bacteria, which shatters on the floor. Which of the following best describes what you should do?

Soak the spill with disinfectant for at least 20 minutes, then dispose of the broken glass in a designated container for broken glass.

While working with a liquid bacterial culture, you get some culture on your gloves. Which of the following best describes what you should do?

Remove your gloves, and place them in the autoclave bag. Then put on a new pair.

What should you do with a plate of bacteria that will NOT be used anymore?

Place it in the appropriate receptacle for autoclaving

What is the primary purpose of keeping doors and windows closed during the laboratory session?

To reduce the incidence of contamination from microbes traveling in the air

After using an inoculating loop or needle to transfer a culture from one medium to the next, what step should be taken next to avoid contamination?

Heat the inoculating loop or needle with the Bunsen burner flame until it becomes red hot

When should lab bench tops be cleaned with disinfectant solution?

At the beginning and at the end of laboratory session

It is especially important to use rapid and efficient manipulation to prevent reproductive spore dissemination when handling which type of culture?

Fungal culture

Which of the following regulations should be observed in order to avoid injury and infection?

Wash hands with detergent and ethyl alcohol, tie back long hair, and wear closed toed shoes while in the laboratory.

If you happen to spill a bacterial culture on the floor during lab, which of the following statements describes the correct clean-up procedure?

Immediately cover the spill with paper towels, saturate with disinfectant, then let sit for 20 minutes.

It is very important that microbiology students must develop aseptic techniques in order to prepare pure cultures. Which of the following statements properly defines aseptic technique?

A technique that is free from contaminating organisms

Which of the following laboratory safety statements is incorrect?

Eating and drinking is allowed in the laboratory as long as open cultures are not on the bench top.

Which of the following statements describes the purpose behind the general rules and regulations of the lab?

To prevent accidental injury and infection of yourself and others

Which of the following is an example of proper laboratory technique?

Wear disposable gloves when working with blood, serum, and other body fluids

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