VCL HW 15 Protein Assays and Hepatobiliary Function Tests

Altered hepatic synthesis, altered protein distribution, and altered protein breakdown and excretion, and by dehydration and overhydration.

Give six factors that may affect total protein concentration.

Albumin is one of the most important proteins in plasma or serum and is the major binding and transport protein in the blood. It is responsible for maintaining the osmotic pressure of the plasma

What is “albumin”? Describe the role that albumin plays

by determining the difference between the total protein and albumin concentrations

Explain how we normally estimate the concentration of globulin.

Bilirubin is an insoluble molecule derived from the breakdown of hemoglobin by macrophage in the spleen. The molecule is bound to albumin and transported to the liver.

What is “bilirubin”? Describe how bilirubin is transported.

to determine the cause of jaundice, to evaluate liver function, and to check the patency of bile duct.

Give three conditions that can be determined by assaying bilirubin.

transferase enzymes alanine transaminase, aspartate transaminase, and the dehydrogenase enzymes iditol dehydrogenase and glutamate dehydrogenase

List the four “leakage enzymes”?