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noncompetitive inhibitor
binds outside active site; changes shape
substrate
reactant molecule for enzyme
plasmolysis
plant cell shrinks from wall
receptor-mediated endocytosis
uptake via specific receptors
Sodium-potassium pump
active transport; pumps Na+ out, K+ in
selective permeability
only some molecules can pass
proton pump
active transport of H+ ions
tonicity
ability of solution to affect cell water balance
transport proteins
helps solute cross membrane
turgid
firm plant cell (hypotonic)
activation energy
energy needed to start a reaction
allosteric regulation
enzyme regulation at site other than active site
active site
enzyme region where substrate binds
anabolic pathway
builds molecules; requires energy
catabolic pathway
breaks down molecules; releases energy
catalyst
speeds up reactions without being consumed
chemical energy
energy stored in bonds
coenzyme
organic enzyme helper (vitamin)
cofactors
non-protein helper (metal ion)
competitive inhibitor
binds active site, blocks substrate
cooperativity
substrate binding increases activity at other sites
endergonic reaction
requires energy; ΔG > 0
enzyme
protein catalyst
enzyme-substrate complex
enzyme bound to substrate
exergonic reaction
releases energy; ΔG < 0
feedback inhibition
end product inhibits pathway
free energy (ΔG)
energy available to do work
induced fit
enzyme changes shape slightly to fit substrate
metabolic pathway
sequence of enzyme reactions
metabolism
total of all enzyme reactions
transport vesicle
carries molecules between organelles
vacuole
storage; central vacuole in plants (water, ions, waste)
vesicle
small transport/storage sac
active transport
requires energy; moves against gradient
amphipathic molecule
both hydrophobic and hydrophilic (phospholipid)
aquaporin
channel protein for water
concentration gradient
difference in solute concentration
cotransport
one solute's downhill movement drives another uphill
diffusion
passive spread of molecules
electrochemical gradient
gradient of ions (concentration + charge)
endocytosis
taking in molecules by vesicle
exocytosis
secretion by vesicles fusing with membrane
facilitated diffusion
passive transport via protein channels/carriers
flaccid
limp plant cell (isotonic)
fluid mosaic model
membrane structure of lipids, proteins, and carbs
gated channel
ion channel that opens/closes in response to stimuli
hypertonic
more solute; cell loses water
hypotonic
less solute; cell gains water
integral protein
penetrates bilayer
ion channel
protein allowing ions to cross
isotonic
equal solute; no net water movement
ligand
signaling molecule that binds receptor
membrane potential
voltage across membrane
osmoregulation
control of solute/water balance
osmosis
diffusion of water
passive transport
no energy needed
peripheral protein
loosely bound to membrane surface
phagocytosis
"cell eating"; engulfing particles
pinocytosis
"cell drinking"; fluids taken in
cell wall
rigid layer in plants, fungi, some prokaryotes; gives structure + protection
chloroplast
organelle for photosynthesis; has thylakoids, stroma, grana
chromatin
uncondensed DNA + protein in nucleus
chromosomes
condensed chromatin; has genetic info
cytoplasm
cytosol + organelles but nucleus
cytoskeleton
protein fibers (microtubules, microfilaments, intermediate filaments) that support, shape, + move cell
cytosol
semifluid in cytoplasm
electron microscopes
high-resolution microscope using electron beams
endomembrane system
has nuclear envelope, ER, golgi apparatus, lysosomes, vacuoles, plasma membrane
rough ER
protein synthesis
smooth ER
lipid synthesis, detox, carbohydrate storage
eukaryotic cell
has nucleus + membrane-bound organelles
extracellular matrix
glycoprotein-rich network in animal cells for signaling, support
gap junction
animal cell communication channel
glycoprotein
protein with carbohydrate chain; cell recognition/signaling
golgi apparatus
packages, sorts, ships proteins + lipids
light microscope
visible light; up to 1000x magnification
lysosome
digestive organelle with hydrolytic enzymes
mitochondria
site of cellular respiration + ATP production
nuclear envelope
double membrane around nucleus, with pores
nucleoid
DNA region in prokaryotes (no membrane)
nucleolus
dense region inside nucleus; makes rRNA + ribosome subunits
nucleus
stores DNA, directs cell activity
organelle
membrane-bound structure with specific function
plasma membrane
selective barrier; phospholipid bilayer
plasmodesmata
channels between plant cells
prokaryotic cells
lacks nucleus & organelles
ribosome
site of protein synthesis (free or bound)