production of monoclonal antibodies

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6 Terms

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monoclonal antibodies

obtained from clones of single B cells

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myeloma cells

grow and divide indefinitely but do not produce antibodies

3
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hybridomas

fused product of myeloma and B cells

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step 1

inject mouse with antigen for which antibodies are desired…allow for immune response to take place and plasma to start producing antibodies…extract plasma cells from mouse

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step 2

fuse plasma cells with myeloma cells…seperate resulting hybridomas and allow growth and division to produce antibodies

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step 3

remove antibodies and test effectiveness to antigen…place producing antibodies into industrial fermenter…harvest and purify monoclonal antibodies