production of monoclonal antibodies
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Last updated 8:40 AM on 1/8/26
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6 Terms
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monoclonal antibodies
obtained from clones of single B cells
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myeloma cells
grow and divide indefinitely but do not produce antibodies
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hybridomas
fused product of myeloma and B cells
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step 1
inject mouse with antigen for which antibodies are desired…allow for immune response to take place and plasma to start producing antibodies…extract plasma cells from mouse
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step 2
fuse plasma cells with myeloma cells…seperate resulting hybridomas and allow growth and division to produce antibodies
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step 3
remove antibodies and test effectiveness to antigen…place producing antibodies into industrial fermenter…harvest and purify monoclonal antibodies
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Flashcards (45)