Molecular Polarity and Chromatography Lab Exercise 6

Vocabulary flashcards covering key terms related to molecular polarity and chromatography from the lab exercise.

Molecular Polarity

Measured by the molecular dipole moment and indicates the distribution of electrical charge within a molecule.

Thin-Layer Chromatography (TLC)

A technique for separating compounds based on their interaction with a stationary phase (TLC plate) and a mobile phase (solvent).

Retention Factor (Rf)

The ratio of the distance traveled by the solute to the distance traveled by the solvent from the index mark in chromatography.

Stationary Phase

The phase in chromatography that does not move, typically a solid adsorbent on which separation occurs.

Mobile Phase

The solvent or mixture of solvents that moves through the stationary phase in chromatography.

Electrostatic Potential Maps

Visual representations that show the distribution of electrostatic potential energy within a molecule, helping to identify partial charges.

Solvent Elution

The process by which the solvent moves up the TLC plate, carrying soluble molecules with it.

Adsorbent

The solid material used in TLC that interacts and retains the compounds being separated.

Factors affecting Rf

Include polarity of the compound, polarity of the solvent, and polarity of the adsorbent.

Destructive Visualization Methods

TLC visualization techniques that involve a chemical reaction with the components on the TLC plate.