II : Blood Preservation

PVC-DEHP (di-ethylhexyl-phthalate)

PVC-DnDP (di-N-decyl phthalate)

Blood Bag material is composed of - for primary bag & - for secondary bags.

Blood bag

- Allow for gas exchange to minimize drastic drops in unit pH.

16 – 18

Needle gauges used for Blood bags is

1. Citrate

2. Phosphate Buffer

3. Dextrose

4. Adenine

Blood Preservation

Citrate

-        Main anticoagulant

Citrate

Binds Ca (to prevent blood clotting)

Phosphate Buffer

-        For ↑ ATP

Phosphate Buffer

↑ATP= ↑production of energy

Dextrose

• Glucose: energy (food) for RBC

Adenine

- For improved survival of RBCs

1. Acid-citrate-dextrose (ACD)

2. Acid-citrate-dextrose (formula A) (ACD-A)

3. Citrate-phosphate-dextrose (CPD)

4. Citrate-phosphate-dextrose-adenine (CPDA-1)

5. Citrate-phosphate-double dextrose (CP2D)

6. Citrate-phosphate-dextrose-adenine-two (CPD-A2)

Approved Preservatives

Acid-citrate-dextrose

Approved Preservatives

ACD

21

Days of preservation of Acid-citrate-dextrose

Acid-citrate-dextrose (formula A)

Approved Preservatives

ACD-A

21

Days of preservation of Acid-citrate-dextrose (formula A)

Citrate-phosphate-dextrose

Approved Preservatives

CPD

21

Days of preservation of Citrate-phosphate-dextrose

Citrate-phosphate-dextrose-adenine

Approved Preservatives

CPDA-1

35

Days of preservation of Citrate-phosphate-dextrose-adenine

Citrate-phosphate-double dextrose

Approved Preservatives

CP2D

21

Days of preservation of Citrate-phosphate-double dextrose

Citrate-phosphate-dextrose-adenine-two

Approved Preservatives

CPD-A2

42

Days of preservation of Citrate-phosphate-dextrose-adenine-two

·    A - Adsol (AS – 1)

·    N - Nutricel (AS – 3)

·    O - Optisol (AS – 5)

·    S - SOLX (AS – 7)

·    S - SAGM

• ·   Saline

• ·   Adenine

• ·   Glucose

• ·   Mannitol

Additive Solutions

42 days

Additive Solutions

Storage period (days) of Adsol (AS-1)

42 days

Additive Solutions

Storage period (days) of Nutricel (AS-3)

42

Additive Solutions

Storage period (days) of Opticel (AS-3)

42

Additive Solutions

Storage period (days) of SOLX (AS-7)

6.6 pH

Additive Solutions

pH (measured at 37°C) of Adsol (AS-1)

6.5 pH

Additive Solutions

pH (measured at 37°C) of Nutricel (AS-3)

6.5 pH

Additive Solutions

pH (measured at 37°C) of Opticel (AS-3)

6.6 pH

Additive Solutions

pH (measured at 37°C) of SOLX (AS-7)

↓

Biochemical Changes Occurring During RBC Storage

pH

↑

Biochemical Changes Occurring During RBC Storage

ATP

↓

Biochemical Changes Occurring During RBC Storage

2,3 DPG

↑

Biochemical Changes Occurring During RBC Storage

Plasma Hemoglobin (Hgb)

↑

Biochemical Changes Occurring During RBC Storage

Plasma Potassium (K)

↓

Biochemical Changes Occurring During RBC Storage

Plasma Sodium (Na)

5000g for 5 mins

Centrifugation

Heavy / Hard Spin for PRBC

5000g for 5 mins

Centrifugation

Heavy / Hard Spin for Platelet concentrate

5000g for 7 mins

Centrifugation

Heavy / Hard Spin for Cryoprecipitate

5000g for 7 mins

Centrifugation

Heavy / Hard Spin for cell free plasma

1. Whole Blood (WB)

2. Packed RBC (pRBC)

3. Platelet-Rich Plasma (PRP)

4. Platelet Concentrate

5. Fresh Frozen Plasma (FFP)

6. Cryoprecipitate

7. Cryosupernate

Blood Components

T

A single blood donation can provide transfusion therapy to multiple px (t/f)

Whole blood

Transfusion of this blood component will provide both O2 carrying capacity & volume expansion

28 days

The expiration of date irradiated RBCs is __ days from the time of irradiation or the original outdate, whichever is sooner.

99.9%

Prestorage leukoreduction using special filters procure at least a __& removal of leukocytes by employing multiple layers of polyester or cellulose acetate nonwoven fibers that trap leukocytes & platelets but allows RBCs to flow through.

high glycerol (40% w/v)

This method in Frozen, deglycerolized RBCs uses a mechanical freezer that provides storage at -80 degree Celsius, and it is the most widely used.

5 days

Platelet concentrates obtain / produced from routine blood donation and or by apheresis stored at RT with continuous agitation is to have at least how many days of shelf-life?

T

Modified True or False:

Statement 1: Platelet can be leukoreduced to help prevent febrile non hemolytic reactions.

Statement 2: Apheresis equipment is designed to produce a leukoreduce product with or without an integrated filter.

Primary bag

Blood bag material such as di-ethvlhexyl-phthalate is for:

primary bag

Blood bag material such as di-ethvlhexyl-phthalate is for:

AS-5

Also known as Optisol:

decreased

Biochemical changes that occur when BC component is stored for 2, 3 DPG.

Increased

Biochemical changes that occur when BC component is stored for plasma potassium

Cryoprecipitate

This blood component is use for blood transfusion therapy for patient with: Hemophilia A, von Willebrand disease, and hypofibrinogemia.

citrate

Odd out from rejuvenation solutions

phosphate

inosine

adenine

citrate

Factor VIII concentrate

Other non-cellular blood product/s that prevent or control bleeding in hemophilia A patients:

Whole Blood (WB)

Collected by venipuncture into blood bags with anticoagulant-preservatives

450 - 500 mL

Volume of Whole Blood (WB)

1 - 6°C BB ref

Storage conditions of Whole Blood (WB)

depends on type of anticoagulant-preservatice

expiry of Whole Blood (WB)

ABO/Rh & crossmatch compatible

transfusion criteria of Whole Blood (WB)

Whole Blood (WB)

Blood placement for active bleeding with blood loss of about 2L or more

Packed RBC (pRBC)

-        QC: Units must have Hct of 65 – 80% 

- QC: Units must have Hct of 65 – 80%

QC of Packed RBC (pRBC)

Packed RBC (pRBC)

- Prepared from WB by light spin & removal of 200 – 250mL of plasma

Packed RBC (pRBC) is prepared from WB by light spin & removal of 200 – 250mL of plasma

Packed RBC (pRBC) is prepared from WB by light spin & removal of 300 – 550mL of plasma (t/f)

• 1 – 6 °C BB Refrigerator

• 24hrs at BB refrigerator if punctured (open system)

Expiry same with WB from which it was prepared

Storage conditions of Packed RBC (pRBC)

• 24hrs at BB refrigerator if punctured (open system)

Storage conditions of Packed RBC (pRBC)

how many hrs at BB refrigerator if punctured (open system)

T

Storage conditions of Packed RBC (pRBC) (t/f)

Expiry same with WB from which it was prepared

Packed RBC (pRBC)

Symptomatic anemia not corrected by Vit. B12, folic acid/iron

Vit. B12, folic acid/iron

Packed RBC (pRBC) (t/f)

Symptomatic anemia not corrected by Vit. -

ABO/Rh & crossmatch compatible

Transfusion Criteria of Packed RBC (pRBC)

Platelet-Rich Plasma (PRP)

- Prepared from WB after preparation of pRBC

6hrs

Platelet-Rich Plasma (PRP) is prepared within - of WB collection

Platelet-Rich Plasma (PRP) & Platelet Concentrate

- Prepared within 6hrs of WB collection

- Light Spin: 2500 rpm for 10mins

Light Spin of Platelet-Rich Plasma (PRP)

200 – 250 mL

Volume of Platelet-Rich Plasma (PRP)

20 – 25 °C RT

Storage condition of Platelet-Rich Plasma (PRP)

Constant agitation

Storage condition of Platelet-Rich Plasma (PRP)

what is needed

Expiry is 5 days from fresh WB collection

Expiry of Platelet-Rich Plasma (PRP)

Platelet-Rich Plasma (PRP)

Treatment & prevention of bleeding due to thrombocytopenia (now replaced by use of plt concentration)

thrombocytopenia

Platelet-Rich Plasma (PRP) Primary use: Treatment & prevention of bleeding due to - (now replaced by use of plt concentration)

ABO compatible

Transfusion criteria of Platelet-Rich Plasma (PRP)

Platelet Concentrate

- Prepared from PRP after heavy spin & removal of plasma

T

Platelet Concentrate is prepared within 6hrs of WB collection (t/f)

3600 rpm for 5 mins

Platelet Concentrate

Heavy Spin: -

50 – 70 mL

Volume of Platelet Concentrate from random donor

300 mL

Volume of Platelet Concentrate from pheresis donor

• 20 – 25 °C RT

• Constant agitation is needed

(same with Platelet-Rich Plasma (PRP))

Storage conditions of Platelet Concentrate

F

Constant agitation is needed

Platelet Concentrate doesn’t need constant agitation (t/f)

Expiry is 5 days from fresh WB collection

(same with Platelet-Rich Plasma (PRP))

expiry of Platelet Concentrate

Platelet Concentrate

Treatment & prevention of bleeding due to thrombocytopenia

ABO compatible

Transfusion criteria of Platelet Concentrate

Fresh Frozen Plasma (FFP)

- Prepared from PRP by freezing

Fresh Frozen Plasma (FFP)

- Prepared within 6 – 8hrs of WB collection

200 - 250 mL

Volume of Fresh Frozen Plasma (FFP)

1 year

Storage conditions of Fresh Frozen Plasma (FFP)

• Expiry is - from fresh WB collection

7 years

Storage conditions of Fresh Frozen Plasma (FFP)

• Expiry is - from fresh WB collection at –65°C BB refrigerator