Protozoa (Amoebae)

Lobosea

Amoebae

Entamoeba histolytica trophozoite

1 nuclei, small karysome w/chromatin, RBC cytoplasmic inclusion

Entamoeba histolytica cyst

1, 2, or 4 nuclei. Small karysome w/chromatin. Cigar shape body

Entamoeba histolytica disease names

amebiasis, amebic dysentery, amebichepatitis

Entamoeba histolytica transmission

ingest infective cysts

Entamoeba histolytica distribution

worldwide

Entamoeba histolytica sample/size

feces/10-20

Entamoeba dispar (commensal)

identical to E. histolytica except there are no RBC ingested

Entamoeba dispar disease name

nonpathogenic

Entamoeba dispar lab report

Entamoeba species unable to diff without presence of RBCs

Entamoeba dispar size

cysts <10; trophs <12

Entamoeba coli (E. coli, commensal)

large karyosome, irregular chromatin clumping

Entamoeba coli disease

nonpathogenic

Entamoeba coli trophs

granular cytoplasm, ingested bacteria

Entamoeba coli cyst

up to 8 nuclei, chromatid bars w/pointed ends

Entamoeba polecki (commensal)

1 nucleus in mature cysts, large glycogen, pointed chromatin

Entamoeba polecki disease

nonpathogenic

Entamoeba gingivalis (commensal)

no cyst stage, ingest WBC,

Blasotcystis hominis (Blastocystis, commensal)

oval, cyst-like, central body,pushed out nuclei

Blasotcystis hominis differences

strictly anaerobic intestinal protozoan

Blasotcystis hominis transmission

fecal oral, contaminated food/water

Blasotcystis hominis sample

fecal, only use saline

Endolimax nana (E. nana, commensal)

smallest (6-12), large irregular karyosome, 4 nuclei

Endolimax nana disease?

common nonpathogenic in intestinal tract

Iodamoeba butschlii (I.butschlii, commensal)

single nucleus, large karyosome, glycogen vacuole

Iodamoeba butschlii disease?

common nonpathogenic in intestinal tract

Acanthamoeba spp

trophs and cysts from brain/skin biopsy

Acanthamoeba spp disease?

opportunistic pathogen

Acanthamoeba spp transmission

troph enters broken skin, respiratory tract, mucous membranes

Acanthamoeba spp disease name

granulomatous amebic encephalitis (GAE)

Acanthamoeba spp common cause

poor contact lens care; swimming w/ contacts in contaminated water

Acanthamoeba spp onset

SLOW, weeks-years

Naegleria fowleri

active trophs in spinal fluid, or brain at autopsy

Naegleria fowleri disease?

opportunistic pathogen, most cases have no disease

Naegleria fowleri disease name

primary amebicmeninogoencephalitis (PAM)

PAM mortality

ends in death after 3-6 days

Naegleria fowleri transmission

troph enters nasal cavity while swimming