TX RXNS meow

benign cold antibodies: what kind of enzyme treatment is likely to detect benign cold antibodies; IgM; Anti-I, anti-IH, anti-i etc

ficin treatment

most common benign cold ab

anti-I

DAT result for benign cold ab

DAT neg

at what point in testing do cold antibodies usuallly cause testing problems

is; abo

pathologic cold antibody is almost always what ab?

anti-I

T/f hemoglobinuria is sometimes seen in those with pathological cold antibodies

t

what diseases/ disorders is due to benign cold antibody

• jaundice

• raynauds phenomenon

DAT result in pathological cold antibody

DAT POS DUE TO COMPLEMENT****

CHD/ cold hemagglutinin disease most often affects what population

those over 50

PHC or paroxysmal cold hemoglobinuria mostly affects who

younger individuals

is autoanti-P IgG or IgM

IgG (is biphasic. during winter gets cold, goes back into body/ away from extremities and then hemolizes)

what is the ab causing paroxysmal cold hemoglobinuria

donath landsteiner ntibody of auto anti-P

What test confirms paroxysmal cold hemoglobinuria

donath landsteiner test

what blood product is used for the donath landsteiner test

PATIENTS SERUM (MUST REQUEST CLOTTED SAMPLE! BC CLOTTING REMOVES COMPLEMENT)

what made cells are used for the donath landsteiner test

group o cells that are P1 pos (50% cell suspension)

t/f the DAT for warm antibodies is almost always negative

f (almost always pos)

DAT result for warm hemolytic abs

DAT pos for either IgG or complement

t/f warm autoantibodies may cause false positives when Rh testing

t

what are the 4 mechanisms of drug induced hemolytic anemia

• drug dependent mechanism

• drug adsorption mech

• membrane modification

• autoantibody formation

what drug induced h.a mechanism: drugs combined w plasma proteins to form immunogens

• drug dependent mechanism

what leads rbcs to lysis during the drug dependent mechanism

activation of complement cascade

drug adsorption mechanism is most commonly found in patients taking what medication

penicillin

what is the DAT result for drug adsorption mechanism

dat reactive with POLY AHG, AND anti-IgG

How much drug do u eed to injest for drug adsorption mechanism to occur

large amt

what medication is most often responsible for membrane modification

cephalosporins

dat result for membrane modification

dat pos w anti-IgG and complement

what drug is most often the culprit for autoantibody formation

alpha-methyldopa/ aldomet (hypertension medication)

what drug induced hemolytic anemia is indistinguishable from WAIHA

autoantibody formation

c

b (bc they have less H antigen)

b

c

*******

c

d (cells were alr coated; implied that it is an utoab bc it is from absorbed serum)

d

c

b

a

d

b

b

d (thinks it is a real antibody)

a