Biology 102 Lab exam 1 review

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If you were working in a biological lab with several chemicals out of these which of the following practices should you employ?

A. Carefully label any bottles you add chemicals to with every component you added (i.e. all the substances in the bottle are included in the label)

B. Wear appropriate PPE including a lab coat, gloves, and safety glasses

C. Be aware of where the first aid kit, emergency eyewash station, and safety shower stations are located

D. Always smell and taste the chemicals before using to ensure the contents of the bottle match the label

E. Treat only hazardous chemicals as hazardous

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1

If you were working in a biological lab with several chemicals out of these which of the following practices should you employ?

A. Carefully label any bottles you add chemicals to with every component you added (i.e. all the substances in the bottle are included in the label)

B. Wear appropriate PPE including a lab coat, gloves, and safety glasses

C. Be aware of where the first aid kit, emergency eyewash station, and safety shower stations are located

D. Always smell and taste the chemicals before using to ensure the contents of the bottle match the label

E. Treat only hazardous chemicals as hazardous

A,B,C

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2

A low pH level indicates that a solution/substance is an

acidic

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3

A high pH indicates that a substance is a

base

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4

Which statement below is true, concerning a conclusion we came to about the three vials of clear fluid we observed in week 2?

A. The vial labeled as vinegar had a pH of 7

B. The vial labeled as vinegar had a pH of 2

C. The vial labeled as vinegar had a pH of 10

B

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5

Which of the following are fundamental characteristics shared by ALL living things?

A. Require energy to sustain themselves

B. Acquire energy from other organisms to sustain themselves

C. Are comprised of multiple cells organized into tissues and organisms

D. Reproduced and pass on characteristics to a new generation

A,B,C,D

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6

When conducting an experiment following the scientific method, how many independent variables can you test at the same time?

One

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7

All organisms are composed of combinations of just four classes of organic molecules (biomolecules). What are these 4 classes of molecules?

nucleic acids, lipids, carbohydrates, and proteins

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8

All 4 classes of organic molecules contain (BLANK) atoms that are linked together to form chains or rings.

carbon

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9

All (BLANK) are reducing sugars

monosaccharides

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10

What are Carbohydrates “building block” or “smallest unit”?

Saccarides/sugars

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11

What are Lipids “building blocks” or “smallest unit”?

Fatty acids

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12

What are proteins “building block” or “smallest unit”?

amino acids

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13

Proteins are incredibly important for living organisms as they play several key roles in sustaining life. Which of the following are functions and/or roles that proteins play?

A. Regulate cell processes

B. Provide structural support, like collagen

C. Store amino acids for developing organisms, like albumin in egg whites

D. Transport key substances throughout an organism, such as hemoglobin carrying oxygen throughout your body

A,B,C,D

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14

Amino acids are linked together by (BLANK) bonds to form polypeptides, and thus proteins/

peptide

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15

If you are looking through a compound light microscope that has a 10x magnification in the oculars, and you have the 40x magnification objective lens in place, what is the total magnification that you are looking at?

400

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16

When conducting any of the biochemistry tests that we performed to test for different types of organic molecules, (biuret’s, lugol’s, benedicts’, etc…) we looked for a change in the (BLANK) to indicate a positive result

color of the regent

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17

As you go from low power lens (the 4x lens) to the high power lens (the 40x lens), what happens to the following attributes with either “increases” or “decreases” as you move from the 4x lens to the 40x lens.

A. Field of view (increase/decrease?)

B. Depth of focus (depth of field) (increase/decrease?)

C. Working distance (increase/decrease?)

D. Light intensity (increase/decrease?)

A. Decrease, B. Decrease, C, Decrease, D. Decrease

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18

On a compound light microscope, the “iris diaphragm” (condenser diaphragm) can be opened and closed to adjust the amount of (BLANK) coming up through the object to the stage

light

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19

Cell membranes are primarily made up of which type of molecule?

Phospholipids

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20

Cellular structures that are bound by membranes and distributed throughout the cytoplasm are referred to as (BLANK)

organelles

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21

Cells of the same tissue types in animals all look similar, true or false?

True

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22

Before the stain was added what did the chromoplast look like?

red/orange

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23

Before the stain was added what did the leucoplast look like?

Clear/colorless

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24

Before the stain was added what did the chloroplast look like?

green

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25

Plastids are found in both plant and animal cells, true or false?

False

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26

What does the Biurets test for?

peptide bonds/proteins

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27

What does the Lugols test for?

Startch

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28

What does the Benedicts test for?

sugars

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29

What does Ninhydrin test for?

amino acids

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30

When you test for amino acids with the Ninhydrin test what do you use?

Filter paper

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31

What are some monosaccharides?

glucose, fructose, ribose, and galactose

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32

What are some Disaccharides?

Sucrose, maltose, and lactose

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33

What are some Polysaccharides?

Starch, glycogen, cellulose, and chitin

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34

How are Disaccharides formed?

by linking two monosaccharides together by using the dehydration synthesis reaction.

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